caa a22 4500 467881715 CHVBK 20180406152722.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00702-006-0488-4 doi (NATIONALLICENCE)springer-10.1007/s00702-006-0488-4 Compartmental protein expression of Tau, GSK-3β and TrkA in cholinergic neurons of aged rats [Elektronische Daten] [G. Niewiadomska, M. Baksalerska-Pazera, I. Lenarcik, G. Riedel] Summary.: During aging basal forebrain cholinergic neurons (BFCNs) degenerate, and we hypothesize this to be the result of a degeneration of the cytoskeleton. As a corollary, retrograde transport of the complex of nerve growth factor (NGF) and its activated receptor phospho-TrkA (P-TrkA) is impaired. Using immunocytochemistry, we here compare young and aged rat brains in their subcellular localization of NGF and P-TrkA in relation to the compartmentalization of phosphorylation-dependent tau protein isoforms. Despite lower P-TrkA immunoreactivity in cortex and hippocampus of aged rats, NGF immunoreactivity was not altered in these areas, but was significantly lower in aged basal forebrain. In young animals, expression of tau isoforms and glycogen synthase kinase-3β (GSK-3β) was restricted to neuritic structures in cortex, hippocampus, and basal forebrain. In contrast, tau and GSK-3β labeling was confined to cell bodies in aged rats. Since a somatic localization of phospho-tau is indicative of cytoskeletal breakdown, we suggest this to be the mechanism the breakdown of trophic support in aging BFCNs. Springer-Verlag, 2006 Keywords: Aging, cholinergic neurons, NGF, axonal transport, cytoskeleton, tau, neurodegenerative diseases nationallicence Niewiadomska G. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut Baksalerska-Pazera M. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut Lenarcik I. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut Riedel G. School of Medical Sciences, University of Aberdeen, Aberdeen, UK aut Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1733-1746 0300-9564 113:11<1733 2006 113 702 https://doi.org/10.1007/s00702-006-0488-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-006-0488-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niewiadomska G. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut NATIONALLICENCE 700 1- Baksalerska-Pazera M. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut NATIONALLICENCE 700 1- Lenarcik I. Department of Neurophysiology, Nencki Institute, Warsaw, Poland aut NATIONALLICENCE 700 1- Riedel G. School of Medical Sciences, University of Aberdeen, Aberdeen, UK aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1733-1746 0300-9564 113:11<1733 2006 113 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer