caa a22 4500 467881723 CHVBK 20180406152722.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00702-006-0479-5 doi (NATIONALLICENCE)springer-10.1007/s00702-006-0479-5 TNF alpha affects the expression of GFAP and S100B: implications for Alzheimer's disease [Elektronische Daten] [M. M. Edwards, S. R. Robinson] Summary.: Neurodegenerative disorders such as Alzheimer's disease are characterized by increased intracellular and extracellular concentrations of the astrocytic proteins glial fibrillary acidic protein (GFAP) and S100B. The present study examined the potential contribution of tumor necrosis factor alpha (TNFα) to these changes by measuring astrocyte viability along with the intracellular and extracellular expression of GFAP and S100B following exposure to this cytokine. Although TNFα did not affect astrocyte viability, the extracellular levels of both proteins were increased three-fold with associated reductions in immunocytochemical labeling. Springer-Verlag, 2006 Keywords: Astrocytes, TNFα, S100B, GFAP, Alzheimer's disease nationallicence AD : Alzheimer's disease nationallicence AC : ammonium citrate nationallicence BSA : bovine serum albumin nationallicence DMEM : Dulbeccos Modified Eagle Medium nationallicence FBS : fetal bovine serum nationallicence GFAP : glial fibrillary acidic protein nationallicence LDH : lactate dehydrogenase nationallicence PB : phosphate buffer nationallicence PBS : phosphate buffered saline nationallicence TNFα : tumor necrosis factor alpha nationallicence Edwards M. M. School of Psychology, Psychiatry and Psychological Medicine, Monash University, Clayton, Victoria, Australia aut Robinson S. R. School of Psychology, Psychiatry and Psychological Medicine, Monash University, Clayton, Victoria, Australia aut Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1709-1715 0300-9564 113:11<1709 2006 113 702 https://doi.org/10.1007/s00702-006-0479-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-006-0479-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edwards M. M. School of Psychology, Psychiatry and Psychological Medicine, Monash University, Clayton, Victoria, Australia aut NATIONALLICENCE 700 1- Robinson S. R. School of Psychology, Psychiatry and Psychological Medicine, Monash University, Clayton, Victoria, Australia aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1709-1715 0300-9564 113:11<1709 2006 113 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer