caa a22 4500 467881731 CHVBK 20180406152722.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00702-006-0500-z doi (NATIONALLICENCE)springer-10.1007/s00702-006-0500-z Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines [Elektronische Daten] [J. Y. Min, M. H. Park, M. K. Park, K. W. Park, N. W. Lee, T. Kim, H. J. Kim, D. H. Lee] Summary.: Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms Springer-Verlag, 2006 Keywords: Staurosporin, PKC, neurite outgrowth, ROS nationallicence Min J. Y. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut Park M. H. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut Park M. K. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut Park K. W. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut Lee N. W. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut Kim T. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut Kim H. J. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut Lee D. H. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1821-1826 0300-9564 113:11<1821 2006 113 702 https://doi.org/10.1007/s00702-006-0500-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-006-0500-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Min J. Y. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut NATIONALLICENCE 700 1- Park M. H. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park M. K. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park K. W. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lee N. W. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut NATIONALLICENCE 700 1- Kim T. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut NATIONALLICENCE 700 1- Kim H. J. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea aut NATIONALLICENCE 700 1- Lee D. H. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1821-1826 0300-9564 113:11<1821 2006 113 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer