caa a22 4500 467881839 CHVBK 20180406152722.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00702-006-0569-4 doi (NATIONALLICENCE)springer-10.1007/s00702-006-0569-4 Differentiated effect of ageing on the enzymes of Krebs' cycle, electron transfer complexes and glutamate metabolism of non-synaptic and intra-synaptic mitochondria from cerebral cortex [Elektronische Daten] [R. F. Villa, A. Gorini, S. Hoyer] Summary.: The effect of ageing on the activity of enzymes linked to Krebs' cycle, electron transfer chain and glutamate metabolism was studied in three different types of mitochondria of cerebral cortex of 1-year old and 2-year old male Wistar rats. We assessed the maximum rate (Vmax) of the mitochondrial enzyme activities in non-synaptic perikaryal mitochondria, and in two populations of intra-synaptic mitochondria. The results indicated that: (i) in normal, steady-state cerebral cortex the values of the catalytic activities of the enzymes markedly differed in the various populations of mitochondria; (ii) in intra-synaptic mitochondria, ageing affected the catalytic properties of the enzymes linked to Krebs' cycle, electron transfer chain and glutamate metabolism; (iii) these changes were more evident in intra-synaptic "heavy” than "light” mitochondria. These results indicate a different age-related vulnerability of subpopulations of mitochondria in vivo located into synapses than non-synaptic ones. Springer-Verlag, 2006 Keywords: Brain energy metabolism, enzymes, Krebs' cycle, electron transfer chain, glutamate metabolism, non-synaptic and intra-synaptic mitochondria, ageing nationallicence Villa R. F. Division of Pharmacology and Pharmacological Biotechnologies, Department of Physiological-Pharmacological Cellular-Molecular Sciences, Laboratory of Neurochemistry and Molecular Medicine, University of Pavia, Pavia, Italy aut Gorini A. Division of Pharmacology and Pharmacological Biotechnologies, Department of Physiological-Pharmacological Cellular-Molecular Sciences, Laboratory of Neurochemistry and Molecular Medicine, University of Pavia, Pavia, Italy aut Hoyer S. Department of Pathology, University of Heidelberg, Heidelberg, Germany aut Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1659-1670 0300-9564 113:11<1659 2006 113 702 https://doi.org/10.1007/s00702-006-0569-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-006-0569-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villa R. F. Division of Pharmacology and Pharmacological Biotechnologies, Department of Physiological-Pharmacological Cellular-Molecular Sciences, Laboratory of Neurochemistry and Molecular Medicine, University of Pavia, Pavia, Italy aut NATIONALLICENCE 700 1- Gorini A. Division of Pharmacology and Pharmacological Biotechnologies, Department of Physiological-Pharmacological Cellular-Molecular Sciences, Laboratory of Neurochemistry and Molecular Medicine, University of Pavia, Pavia, Italy aut NATIONALLICENCE 700 1- Hoyer S. Department of Pathology, University of Heidelberg, Heidelberg, Germany aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer-Verlag 113/11(2006-11-01), 1659-1670 0300-9564 113:11<1659 2006 113 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer