caa a22 4500 467882428 CHVBK 20180406152724.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10393-006-0021-0 doi (NATIONALLICENCE)springer-10.1007/s10393-006-0021-0 Differential Expression of Immune Response Genes in Steller Sea Lions (Eumetopias jubatus): An Indicator of Ecosystem Health? [Elektronische Daten] [Lizabeth Bowen, Brian Aldridge, Kimberlee Beckmen, Tom Gelatt, Lorrie Rea, Kathy Burek, Ken Pitcher, Jeffrey Stott] Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be used to measure the potential immunologic vigor of a population. However, since ecosystem-induced changes to MHC genotype can be slow to emerge, measurement of differential expression of these genes can potentially provide real-time evidence of immunologic perturbations. MHC DRB genes were cloned and sequenced using peripheral blood mononuclear leukocytes derived from 10 Steller sea lions from Southeast Alaska, Prince William Sound, and the Aleutian Islands. Nine unique DRB gene sequences were represented in each of 10 animals. MHC DRB gene expression was measured in a subset of six sea lions. Although DRB in genomic DNA was identical in all individuals, relative levels of expressed DRB mRNA was highly variable. Selective suppression of MHC DRB genes could be indicative of geographically disparate environmental pressures, thereby serving as an immediate and sensitive indicator of population and ecosystem health. Springer Science+Business Media, Inc., 2006 Steller sea lion nationallicence ecosystem health nationallicence Bowen Lizabeth Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut Aldridge Brian Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut Beckmen Kimberlee Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut Gelatt Tom National Marine Fisheries Service, Alaska Fisheries Science Center/NMML, Seattle, WA aut Rea Lorrie Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut Burek Kathy Alaska Veterinary Pathology Services, Eagle River, AK aut Pitcher Ken Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut Stott Jeffrey Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut EcoHealth Springer-Verlag 3/2(2006-06-01), 109-113 1612-9202 3:2<109 2006 3 10393 https://doi.org/10.1007/s10393-006-0021-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10393-006-0021-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bowen Lizabeth Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Aldridge Brian Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Beckmen Kimberlee Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut NATIONALLICENCE 700 1- Gelatt Tom National Marine Fisheries Service, Alaska Fisheries Science Center/NMML, Seattle, WA aut NATIONALLICENCE 700 1- Rea Lorrie Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut NATIONALLICENCE 700 1- Burek Kathy Alaska Veterinary Pathology Services, Eagle River, AK aut NATIONALLICENCE 700 1- Pitcher Ken Division of Wildlife Conservation, Marine Mammals Section, Alaska Department of Fish and Game, Fairbanks, AK aut NATIONALLICENCE 700 1- Stott Jeffrey Laboratory for Marine Mammal Immunology, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, University of California at Davis, 1 Shields Avenue, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- EcoHealth Springer-Verlag 3/2(2006-06-01), 109-113 1612-9202 3:2<109 2006 3 10393 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer