caa a22 4500 467886229 CHVBK 20180406152735.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10924-005-8710-2 doi (NATIONALLICENCE)springer-10.1007/s10924-005-8710-2 Enzymatic Degradation and Aminolysis of Microbial Poly(3-hydroxybutyrate- co -4-hydroxybutyrate) Single Crystals [Elektronische Daten] [Wen-chuan Hsieh, Hiroshi Mitomo, Ken-ichi Kasuya, Tadashi Komoto] Solution-grown single crystals of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] were hydrolyzed by polyhydroxybutyrate (PHB) depolymerase from Ralstonia pickettii T1. Enzymatic degradation proceeded from the edges of lamellar crystals, yielding serrated contour and small crystal fragments. Gel permeation chromatography analysis revealed that the molecular weights of the crystals decreased during enzymatic degradation, suggesting that the enzymatic hydrolysis of chain-folding regions at the crystal surfaces occurred in addition to the enzymatic degradation at crystal laterals or edges. After P(3HB-co-4HB) single crystals were aminolysed in 20% aqueous methylamine solution to remove the folded-chain regions and enzymatic degradation by lipase from Rhizopus oryzae to remove 4HB components at crystal surfaces of single crystal aminolyzed, it was found that a small amount (up to ca. 2mol%) of 4HB component can be incorporated into the P(3HB) mother crystal lattice irrespective of the 4HB content. Springer Science+Business Media, Inc., 2006 Microbial poly(3-hydroxybutyrate- co -4-hydroxybutyrate) nationallicence single crystal nationallicence enzymatic degradation nationallicence aminolysis nationallicence Hsieh Wen-chuan Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut Mitomo Hiroshi Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut Kasuya Ken-ichi Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut Komoto Tadashi Department of Chemistry, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers 14/1(2006-01-01), 79-87 1566-2543 14:1<79 2006 14 10924 https://doi.org/10.1007/s10924-005-8710-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-005-8710-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hsieh Wen-chuan Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut NATIONALLICENCE 700 1- Mitomo Hiroshi Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut NATIONALLICENCE 700 1- Kasuya Ken-ichi Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut NATIONALLICENCE 700 1- Komoto Tadashi Department of Chemistry, Faculty of Engineering, Gunma University, 376-8515, Kiryu, Gunma, Japan aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers 14/1(2006-01-01), 79-87 1566-2543 14:1<79 2006 14 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer