caa a22 4500 467886342 CHVBK 20180406152735.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s10924-006-0016-5 doi (NATIONALLICENCE)springer-10.1007/s10924-006-0016-5 Comparing the Growth Characteristics of Three Bacteria Involved in Degrading Rubbers [Elektronische Daten] [Ji-Dong Gu, Li Pan] Three bacteria isolated for degradation of rubbers were compared for their growth characteristics derived from the Bradford protein assay and turbidity (optical density, OD) measurement. Both Alcaligenes xylosoxidans T2 and Pseudomonas aeruginosa GP10 were fast-growing bacteria while Nocardia corynebacterioides S3 was a slow grower utilizing rubber as the sole source of carbon and energy, but the extent of degradation was lower by the formers than the latter. A. xylosoxidans T2, P. aeruginosa GP10 and N. corynebacterioides S3 showed a typical sigmoidal growth pattern based on binding of Coomassie Brilliant Blue G250 to bacterial proteins and spectrophotometrical measurement at 600nm. Both assays showed similar growth characteristics for all three bacteria in this study. Degradation of rubber was more pronounced by N. corynebacterioides S3 than either A. xylosoxidans T2 or P. aeruginosa GP10 during 70days of incubation. Our results suggest that slow-growing bacteria may play a much greater role in degrading polymeric materials than was previously believed. Springer Science+Business Media, Inc., 2006 Bradford assay nationallicence Coomassie Brilliant Blue-G250 nationallicence Bacterial growth nationallicence Polymer deterioration nationallicence Gu Ji-Dong Marine Surface Biology/Environmental Microbiology, Key Laboratory of Tropical Marine Environment Dynamics (LED), South China Sea Institute of Oceanography, Chinese Academy of Sciences, 164 Xingang Road West, 510301, Guangzhou, P.R. China aut Pan Li Laboratory of Environmental Microbiolgy and Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, P.R. China aut Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/3(2006-07-01), 273-279 1566-2543 14:3<273 2006 14 10924 https://doi.org/10.1007/s10924-006-0016-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-006-0016-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gu Ji-Dong Marine Surface Biology/Environmental Microbiology, Key Laboratory of Tropical Marine Environment Dynamics (LED), South China Sea Institute of Oceanography, Chinese Academy of Sciences, 164 Xingang Road West, 510301, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Pan Li Laboratory of Environmental Microbiolgy and Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, P.R. China aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/3(2006-07-01), 273-279 1566-2543 14:3<273 2006 14 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer