caa a22 4500 46788644X CHVBK 20180406152736.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10924-006-0035-2 doi (NATIONALLICENCE)springer-10.1007/s10924-006-0035-2 Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3 [Elektronische Daten] [Ken'ichiro Matsumoto, Yuko Arai, Rina Nagao, Takaaki Murata, Kazuma Takase, Hideo Nakashita, Seiichi Taguchi, Hiroaki Shimada, Yoshiharu Doi] In this paper, the photosynthetic production of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymers is reported. The wild-type and highly active doubly mutated PHA synthase 1 (S325T/Q481K, abbreviated ST/QK) genes from Pseudomonas sp. 61-3 were introduced into Arabidopsis thaliana. Peroxisome targeting signal 1 (PTS1) was used to target PHA synthases into the peroxisome to synthesize PHA from the intermediates of the β-oxidation pathway. The transgenic Arabidopsis produced PHA copolymers consisting of 40-57mol% 3-hydroxybutyrate, 21-49mol% 3-hydroxyvalerate, 8-18mol% 3-hydroxyhexanoate, and 2-8mol% 3-hydroxyoctanoate. The maximum PHA contents were 220μg/g cell dry weight (cdw) in leaves, and 36μg/g cdw in stems, respectively. The expression of the ST/QK mutated PHA synthase in leaves gene did not lead to significant difference in PHA content and monomer composition of PHAs, compared to the wild-type PHA synthase gene, suggesting that the supply of monomers may be a rate-determining step of PHA biosynthesis in the peroxisome. However, in stems, there were significant differences dependent on whether the wild-type or ST/QK mutated PHA synthase was expressed. These results suggest that tissue-specific monomer availability is important in determining the final mol% composition of PHA copolymers produced by the peroxisome in plants. Springer Science+Business Media, Inc., 2006 Polyhydroxyalkanoate nationallicence Arabidopsis thaliana nationallicence Peroxisome nationallicence Pseudomonas sp. 61-3 nationallicence 3-hydroxyvalerate nationallicence Matsumoto Ken'ichiro Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Arai Yuko Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut Nagao Rina Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Murata Takaaki Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Takase Kazuma Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut Nakashita Hideo Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut Taguchi Seiichi Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, 060-8628, Sapporo, Japan aut Shimada Hiroaki Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut Doi Yoshiharu Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/4(2006-10-01), 369-374 1566-2543 14:4<369 2006 14 10924 https://doi.org/10.1007/s10924-006-0035-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-006-0035-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsumoto Ken'ichiro Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Arai Yuko Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Nagao Rina Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Murata Takaaki Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Takase Kazuma Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Nakashita Hideo Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Taguchi Seiichi Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, 060-8628, Sapporo, Japan aut NATIONALLICENCE 700 1- Shimada Hiroaki Department of Biological Science and Technology, Tokyo University of Science, Yamazaki 2641, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Doi Yoshiharu Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/4(2006-10-01), 369-374 1566-2543 14:4<369 2006 14 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer