caa a22 4500 467886474 CHVBK 20180406152736.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10924-006-0031-6 doi (NATIONALLICENCE)springer-10.1007/s10924-006-0031-6 Purification and Properties of a Poly (β-hydroxybutyrate) Depolymerase From Penicillium sp [Elektronische Daten] [Hongyu Liu, Hu Zhang, Shan Chen, Dongbo Liu, Hongmei Xia] An extracellular poly (β-hydroxybutyrate) (PHB) depolymerase was purified from a Penicillium sp. DS9701-09a by centrifugation, ultrafiltration, precipitation and gel filtration chromatography. The specific activity of the purified enzyme was 37.9-folds higher than that of the culture supernatant and the recovery yield was 11.8%. The PHB deploymerase molecular mass was 44.8kDa from analysis of both Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Matrix-assisted laser desorption-time-of-flight (MALDI-TOF) mass spectrometer. The isoelectric point of 6.7 for the enzyme was determined by a two-dimensional electrophoresis. The optimum enzyme activity was observed at a temperature of 50°C and pH5.0. The apparent K m of the enzyme was found to be 1.35mg/mL. The PHB depolymerase consisted of 16 kinds of normal amino acids. The secondary structure of the enzyme was determined by CD spectrum. α-helix and β-turn were found to be 66% and 34% for the enzyme without ammonium sulphite. Chemical inhibition on the PHB depolymerase activity was examined and EDTA was found to have a significantly inhibitory effect. Springer Science+Business Media, Inc., 2006 PHB depolymerase nationallicence Purification nationallicence Properties nationallicence PHB hydrolysis nationallicence Penicillium sp nationallicence Liu Hongyu School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut Zhang Hu Department of Biochemical Engineering, University College London, WC1E 7JE, Torrington Place, London aut Chen Shan School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut Liu Dongbo School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut Xia Hongmei School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/4(2006-10-01), 419-426 1566-2543 14:4<419 2006 14 10924 https://doi.org/10.1007/s10924-006-0031-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10924-006-0031-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Hongyu School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut NATIONALLICENCE 700 1- Zhang Hu Department of Biochemical Engineering, University College London, WC1E 7JE, Torrington Place, London aut NATIONALLICENCE 700 1- Chen Shan School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut NATIONALLICENCE 700 1- Liu Dongbo School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut NATIONALLICENCE 700 1- Xia Hongmei School of Life Science, Northeast Normal University, 5268 People's Road, 130024, Changchun, P. R. China aut NATIONALLICENCE 773 0- Journal of Polymers and the Environment Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 14/4(2006-10-01), 419-426 1566-2543 14:4<419 2006 14 10924 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer