caa a22 4500 467888256 CHVBK 20180406152741.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11144-006-0103-6 doi (NATIONALLICENCE)springer-10.1007/s11144-006-0103-6 Enzymes immobilized on montmorillonite: comparison of performance in batch and packed-bed reactors [Elektronische Daten] [Sanjay Gopinath, Sankaran Sugunan] Summary: The enzymes a-amylase, invertase and glucoamylase were immobilized on acid activated montmorillonite using two techniques, viz. adsorption and covalent binding, and their activities were tested in a batch and packed-bed reactor and were compared. The packed-bed reactor showed an improved performance for all immobilized enzymes, which was attributed to lowering of diffusional restrictions to mass transfer. Lower activity in case of batch reactor for immobilized invertase was due to a combined effect of loss of native conformation of enzyme on account of immobilization and mass transfer resistances due to improper diffusion of substrate to the active site of enzyme. For immobilized glucoamylase, the packed-bed reactor demonstrated exceptionally high activity that was very close to the free enzyme. Covalently bound glucoamylase showed higher activity than the free enzyme. Springer-Verlag/Akadémiai Kiadó, 2006 adsorption nationallicence packed-bed reactor nationallicence batch reactor nationallicence covalent binding nationallicence Immobilization nationallicence Gopinath Sanjay Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India aut Sugunan Sankaran Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India aut Reaction Kinetics and Catalysis Letters Kluwer Academic Publishers 88/1(2006-06-01), 3-9 0133-1736 88:1<3 2006 88 11144 https://doi.org/10.1007/s11144-006-0103-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11144-006-0103-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gopinath Sanjay Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India aut NATIONALLICENCE 700 1- Sugunan Sankaran Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India aut NATIONALLICENCE 773 0- Reaction Kinetics and Catalysis Letters Kluwer Academic Publishers 88/1(2006-06-01), 3-9 0133-1736 88:1<3 2006 88 11144 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer