caa a22 4500 467889570 CHVBK 20180406152744.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00049-005-0320-x doi (NATIONALLICENCE)springer-10.1007/s00049-005-0320-x Adaptation of a four-arm olfactometer for behavioural bioassays of large beetles [Elektronische Daten] [Imen Saïd, Rosa Torre, Jean-Paul Morin, Didier Rochat] Summary.: We adapted a four-arm olfactometer (55×55×5.5cm) for bioassays of large insects and its usefulness was evaluated by testing the responses of three beetles, the palm weevil Rhynchophorus palmarum (L.) (Coleoptera: Curculionidea) and two Dynast scarabs Strategus aloeus (L.) and Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) to plant odours and aggregation pheromones. This olfactometer was coupled to a volatile delivery system dispensing highly volatile semiochemicals at constant concentrations. We materialized airflows in the olfactometer by discolouring sensitive papers with acid vapours under various conditions (air straighten by stainless steel grids in inputs and output, 500ml/min per arm) to visualize air turbulence and to test air-tightness. The volatile delivery system can be used to mix and dispense a broad range of concentrations of semiochemicals in air from diluted aqueous solutions. It was calibrated by measuring the release of the pheromone using solid phase-microextraction (SPME). Dose-response curves to synthetic pheromone were obtained for O. rhinoceros of both sexes. Coupling the volatile delivery system to the fourarm olfactometer provided a system with which the responses of R. palmarum and S. aloeus to their synthetic aggregation pheromones and to natural plant volatiles could be readily observed and studied. Birkhäuser Verlag, Basel, 2005 Four-arm olfactometer nationallicence volatile delivery system nationallicence aggregation pheromone nationallicence palm beetles nationallicence Coleoptera nationallicence Curculionidae nationallicence Scarabaeidae nationallicence Rhynchophorus palmarum nationallicence Oryctes rhinoceros nationallicence Strategus aloeus nationallicence Saïd Imen CNRS UMR 6552, Université de Rennes 1, Campus de Beaulieu, 35042, Rennes cedex, France aut Torre Rosa Cenipalma, Calle 21 No. 42C-47, Santa Fé de Bogotá, Colombia aut Morin Jean-Paul UR 31, TA 80/02, Avenue de la Lironde, 34398, Montpellier cedex 5, France aut Rochat Didier Physiologie de l'Insecte: Signalisation et Communication, INRA, UMR 1272, Route de Saint-Cyr, 78026, Versailles cedex, France aut CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/1(2006-03-01), 9-16 0937-7409 16:1<9 2006 16 49 https://doi.org/10.1007/s00049-005-0320-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-005-0320-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saïd Imen CNRS UMR 6552, Université de Rennes 1, Campus de Beaulieu, 35042, Rennes cedex, France aut NATIONALLICENCE 700 1- Torre Rosa Cenipalma, Calle 21 No. 42C-47, Santa Fé de Bogotá, Colombia aut NATIONALLICENCE 700 1- Morin Jean-Paul UR 31, TA 80/02, Avenue de la Lironde, 34398, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Rochat Didier Physiologie de l'Insecte: Signalisation et Communication, INRA, UMR 1272, Route de Saint-Cyr, 78026, Versailles cedex, France aut NATIONALLICENCE 773 0- CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/1(2006-03-01), 9-16 0937-7409 16:1<9 2006 16 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer