caa a22 4500 467889619 CHVBK 20180406152745.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00049-005-0327-3 doi (NATIONALLICENCE)springer-10.1007/s00049-005-0327-3 Furanocoumarins and their detoxification in a tri-trophic interaction [Elektronische Daten] [Jennifer McGovern, Arthur Zangerl, Paul Ode, May Berenbaum] Abstract.: The parsnip webworm, Depressaria pastinacella, specializes on wild parsnip, Pastinaca sativa, and several species of Heracleum, hostplants rich in toxic furanocoumarins. Rates of furanocoumarin metabolism in this species are among the highest known for any insect. Within its native range in Europe, webworms are heavily parasitized by the polyembryonic parasitoid wasp Copidosoma sosares. In this study, we determined whether these parasitoids are exposed to furanocoumarins in host hemolymph, whether they can metabolize furanocoumarins, and whether parasitism influences the ability of webworms to detoxify furanocoumarins. Hemolymph of webworms fed artificial diet containing 0.3 % fresh weight xanthotoxin, a furanocoumarin prevalent in wild parsnip hosts, contained trace amounts of this toxin; as well, hemolymph of webworms consuming P. sativa flowers and fruits contained trace amounts of six of seven furanocoumarins present in the hostplant. Thus, parasitoids likely encounter furanocoumarins in host hemolymph. Assays of xanthotoxin metabolism in C. sosares failed to show any ability to metabolize this compound. Parasitized webworms, collected from populations of Heracleum sphondylium in the Netherlands in 2004, were on average 55 % larger by weight than unparasitized individuals. This weight is inclusive of host and parasitoid masses. Absolute rates of detoxification (nmoles min−1) of five different furanocoumarins were indistinguishable between parasitized and unparasitized ultimate instars, suggesting that the intrinsic rates of metabolism are fixed. Thus, although parasitized larvae are larger, detoxification rates are not commensurate with size; rates in parasitized larvae expressed per gram of larval mass were 25 % lower than in unparasitized larvae. Birkhäuser Verlag, Basel, 2006 Copidosoma sosares nationallicence Depressaria pastinacella nationallicence Pastinaca sativa nationallicence furanocoumarins nationallicence tri-trophic nationallicence detoxification nationallicence hemolymph nationallicence cytochrome P450 nationallicence McGovern Jennifer Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut Zangerl Arthur Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut Ode Paul Department of Entomology, North Dakota State University, 202 Hultz Hall, 1300 Albrecht Blvd., 58105, Fargo, ND, USA aut Berenbaum May Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/1(2006-03-01), 45-50 0937-7409 16:1<45 2006 16 49 https://doi.org/10.1007/s00049-005-0327-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-005-0327-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McGovern Jennifer Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Zangerl Arthur Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Ode Paul Department of Entomology, North Dakota State University, 202 Hultz Hall, 1300 Albrecht Blvd., 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Berenbaum May Department of Entomology, University of Illinois at Urbana-Champaign, 320 Morrill Hall, 505 S. Goodwin, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/1(2006-03-01), 45-50 0937-7409 16:1<45 2006 16 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer