caa a22 4500 467889767 CHVBK 20180406152745.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00049-006-0349-5 doi (NATIONALLICENCE)springer-10.1007/s00049-006-0349-5 Chemosensory and behavioural responses of the turnip sawfly, Athalia rosae , to glucosinolates and isothiocyanates [Elektronische Daten] [Alison Barker, Reitumetse Molotsane, Caroline Müller, Urs Schaffner, Erich Städler] Summary.: The turnip sawfly Athalia rosae sequesters glucosinolates from its cruciferous host plants in the larval stage. Investigation of the chemosensory and behavioural responses of adult A. rosae to glucosinolates and their volatile hydrolysis products, isothiocyanates, revealed that females detect glucosinolates by contact chemoreception and isothiocyanates by antennal olfaction. In electroantennogram recordings, four isothiocyanates (allyl [2-propenyl] isothiocyanate, benzyl isothiocyanate, butyl isothiocyanate and iberverin [3-methylthiopropyl isothiocyanate]) were active at all doses presented, including the lowest (0.1μg), whilst the threshold for detection of three others, iberin [3-methylsulphinylpropyl isothiocyanate], methyl isothiocyanate, and sulforaphane [4-methylsulphinylbutyl isothiocyanate], was higher, at between 1 and 10μg (source concentration of volatiles). Allyl isothiocyanate attracted experienced females in a four-chambered olfactometer, whilst naïve females showed no response. Allyl isothiocyanate also attracted mature females to baited yellow water traps in field trials, although immature females were repelled at high isothiocyanate concentrations. In laboratory behavioural bioassays the glucosinolates sinigrin (allyl [2-propenyl] glucosinolate) and sinalbin (p-hydroxybenzyl glucosinolate), stimulated ovipositor probing in mature female A. rosae to an extent comparable to hot-water extracts of their host plants. These responses show that glucosinolates and isothiocyanates play an important role in host finding and host recognition in A. rosae. Birkhäuser Verlag, Basel, 2006 glucosinolate nationallicence isothiocyanate nationallicence host-finding nationallicence host-recognition nationallicence sawfly nationallicence Athalia rosae nationallicence Delia radicum nationallicence Hymenoptera nationallicence Tenthredinidae nationallicence Diptera nationallicence Brassicaceae nationallicence Barker Alison CABI-Bioscience Switzerland Centre, Rue des Grillons 1, CH-2800, Delémont, Switzerland aut Molotsane Reitumetse Department of Biological Sciences, Imperial College, Silwood Park, SL5 7PY, Ascot, Berkshire, UK aut Müller Caroline Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Julius-von-Sachs-Platz 3, D-97082, Würzburg, Germany aut Schaffner Urs CABI-Bioscience Switzerland Centre, Rue des Grillons 1, CH-2800, Delémont, Switzerland aut Städler Erich Eidg. Forschungsanstalt, CH-8820, Wädenswil, Switzerland aut CHEMOECOLOGY Birkhäuser-Verlag; www.birkhäuser.ch 16/4(2006-12-01), 209-218 0937-7409 16:4<209 2006 16 49 https://doi.org/10.1007/s00049-006-0349-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-006-0349-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barker Alison CABI-Bioscience Switzerland Centre, Rue des Grillons 1, CH-2800, Delémont, Switzerland aut NATIONALLICENCE 700 1- Molotsane Reitumetse Department of Biological Sciences, Imperial College, Silwood Park, SL5 7PY, Ascot, Berkshire, UK aut NATIONALLICENCE 700 1- Müller Caroline Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Julius-von-Sachs-Platz 3, D-97082, Würzburg, Germany aut NATIONALLICENCE 700 1- Schaffner Urs CABI-Bioscience Switzerland Centre, Rue des Grillons 1, CH-2800, Delémont, Switzerland aut NATIONALLICENCE 700 1- Städler Erich Eidg. Forschungsanstalt, CH-8820, Wädenswil, Switzerland aut NATIONALLICENCE 773 0- CHEMOECOLOGY Birkhäuser-Verlag; www.birkhäuser.ch 16/4(2006-12-01), 209-218 0937-7409 16:4<209 2006 16 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer