caa a22 4500 467889775 CHVBK 20180406152745.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00049-006-0348-6 doi (NATIONALLICENCE)springer-10.1007/s00049-006-0348-6 The chelation of metal ions by the acylpolyamine toxins from the web-spider Nephilengys cruentata [Elektronische Daten] effects in the intoxication/detoxification of preys [Maria Manzoli-Palma, Nivar Gobbi, Mario Palma] Summary.: Orb-web-spiders present a series of different strategies for prey capture, involving the use of different types of silk for web building, the use of adhesive traps in the webs, the secretion of toxic compounds to the spider's preys in the adhesive coating of the capture web and the biosynthesis of a wide range of structurally related acylpolyamine toxins in their venoms. The polyamine toxins usually block neuromuscular junctions and/or the central nervous system (CNS) of Arthropods, targeting specially the ionotropic glutamate receptors; this way these toxins are used are as chemical weapons to kill / paralyze the spider's prey. Polyamine toxins contain many azamethylene groups involved with the chelation of metal ions, which in turn can interact with the glutamate receptors, affecting the toxicity of these toxins. It was demonstrated that the chelation of Ni+2, Fe+2, Pb+2, Ca+2 and Mg+2 ions by the desalted crude venom of Nephilengys cruentata and by the synthetic toxin JSTX-3, did not cause any significant change in the toxicity of the acylpolyamine toxins to the model-prey insect (honeybees). However, it was also reported that the chelation of Zn+2 ions by the acylpolyamines potentiated the lethal / paralytic action of these toxins to the honeybees, while the chelation of Cu+2 ions caused the inverse effect. Atomic absorption spectrometry and Plasma-ICP analysis both of N. cruentata venom and honeybee's hemolymph revealed that the spider's venom concentrates Zn+2 ions, while the honeybee's hemolymph concentrates Cu+2 ions. These results are suggesting that the natural accumulation of Zn+2 ions in N. cruentata venom favors the prey catching and/or its maintenance in the web, while the natural accumulation of Cu+2 ions in prey's hemolymph minimizes the efficiency of the acylpolyamine toxins as killing/paralyzing tool. Birkhäuser Verlag, Basel, 2006 Acylpolyamine toxins nationallicence spider venom nationallicence neurotoxicity nationallicence metal ion chelation nationallicence lethality nationallicence paralysis nationallicence Manzoli-Palma Maria Department of Ecology, São Paulo State University (UNESP), CEP 13506-900, Rio Claro, SP, Brazil aut Gobbi Nivar Department of Ecology, São Paulo State University (UNESP), CEP 13506-900, Rio Claro, SP, Brazil aut Palma Mario CEIS/Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP), Rio Claro, SP, Brazil aut CHEMOECOLOGY Birkhäuser-Verlag; www.birkhäuser.ch 16/4(2006-12-01), 203-208 0937-7409 16:4<203 2006 16 49 https://doi.org/10.1007/s00049-006-0348-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-006-0348-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manzoli-Palma Maria Department of Ecology, São Paulo State University (UNESP), CEP 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 700 1- Gobbi Nivar Department of Ecology, São Paulo State University (UNESP), CEP 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 700 1- Palma Mario CEIS/Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP), Rio Claro, SP, Brazil aut NATIONALLICENCE 773 0- CHEMOECOLOGY Birkhäuser-Verlag; www.birkhäuser.ch 16/4(2006-12-01), 203-208 0937-7409 16:4<203 2006 16 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer