caa a22 4500 467889813 CHVBK 20180406152745.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00049-005-0332-6 doi (NATIONALLICENCE)springer-10.1007/s00049-005-0332-6 Wind tunnel attraction of grapevine moth females, Lobesia Botrana , to natural and artificial grape odour [Elektronische Daten] [Marco Tasin, Anna-Carin Bäckman, Marie Bengtsson, Nélia Varela, Claudio Ioriatti, Peter Witzgall] Summary.: Host plant volatiles which attract insect herbivores for egg-laying are of principal interest with respect to insect ecology and evolution. Direct applications concern population monitoring and control through behavioural manipulation. Identification of behaviourally active plant secondary metabolites is essential also for plant breeding for insect resistance. Grapevine moth females Lobesia botrana are attracted by upwind flight to green grape berries Vitis vinifera. The headspace of grape berries was collected on air filters. A solvent extract of these filters, released from a sprayer, attracted females in the wind tunnel. The results demonstrate that volatile cues mediate attraction of grapevine moth females to grape berries, and that headspace collections capture the essence of this odour signal. The air filter extracts were examined by gas chromatography coupled with electroantennographic detection, and the compounds eliciting a consistent antennal response in L. botrana females were identified by mass spectrometry. The headspace collection apparatus was calibrated for collection efficiency of the active compounds. Their recovery rate ranged from 35 % for methyl salicylate to 83 % for (E,E)-α-farnesene. A synthetic ten-component blend was then formulated. The blend consisted of compounds eliciting an antennal response, formulated in a blend ratio corrected for differences in collection efficiency. Subsequent wind tunnel tests showed that female attraction to this synthetic ten-component blend was not significantly different from attraction to grape berries, or to headspace collections of the same berries. At a release rate of 35ng/h of the most abundant compound (E)-β-caryophyllene, 20 % of the test females approached the source of sprayed headspace collections and the ten-component synthetic blend, respectively. In comparison, 100 g of green berries, releasing the main compound (E)-β-caryophyllene at a rate of ca. 4.7ng/h, attracted 10 % of the females by upwind flight followed by source contact. Birkhäuser Verlag, Basel, 2006 Lobesia botrana nationallicence Tortricidae nationallicence Lepidoptera nationallicence plant volatile compounds nationallicence kairomone nationallicence upwind attraction nationallicence wind tunnel nationallicence headspace collection nationallicence Tasin Marco Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut Bäckman Anna-Carin Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut Bengtsson Marie Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut Varela Nélia Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut Ioriatti Claudio Agricultural Research Station, 38 010 San Michele all'Adige, Trento, Italy aut Witzgall Peter Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/2(2006-06-01), 87-92 0937-7409 16:2<87 2006 16 49 https://doi.org/10.1007/s00049-005-0332-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00049-005-0332-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tasin Marco Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Bäckman Anna-Carin Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Bengtsson Marie Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Varela Nélia Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Ioriatti Claudio Agricultural Research Station, 38 010 San Michele all'Adige, Trento, Italy aut NATIONALLICENCE 700 1- Witzgall Peter Department of Crop Science, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden aut NATIONALLICENCE 773 0- CHEMOECOLOGY Birkhäuser-Verlag; www.birkhauser.ch 16/2(2006-06-01), 87-92 0937-7409 16:2<87 2006 16 49 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer