caa a22 4500 467890404 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0248-6 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0248-6 Comparison of the nucleotide and amino acid sequences of parental and attenuated isolates of Zucchini yellow mosaic virus [Elektronische Daten] [Wei-Qin Wang, Tomohide Natsuaki, Yoshitaka Kosaka, Seiichi Okuda] To identify possible sites of viral attenuation, the complete nucleotide sequences of two isolates of Zucchini yellow mosaic virus (ZYMV) were determined; a severe isolate Z5-1 and an attenuated isolate from Z5-1 (designated ZYMV-2002). The viral genome of both isolates consisted of 9593 nucleotides in size and contained an open reading frame encoding a single polyprotein of 3080 amino acids. Comparison of the nucleotide sequences for Z5-1 and ZYMV-2002 revealed 14 nucleotide mutations, resulting in seven amino acid substitutions with four in the HC-Pro region, two in the CI region, and one in the NIb region. These results provide a genetic basis for future manipulation of the ZYMV reverse genetics system. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Zucchini yellow mosaic virus nationallicence Potyvirus nationallicence Sequence nationallicence Attenuation nationallicence Mutation nationallicence Wang Wei-Qin Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut Natsuaki Tomohide Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut Kosaka Yoshitaka Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto, Japan aut Okuda Seiichi Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 52-56 1345-2630 72:1<52 2006 72 10327 https://doi.org/10.1007/s10327-005-0248-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0248-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Wei-Qin Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Natsuaki Tomohide Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Kosaka Yoshitaka Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto, Japan aut NATIONALLICENCE 700 1- Okuda Seiichi Faculty of Agriculture, Utsunomiya University, 350 Mine-Machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 52-56 1345-2630 72:1<52 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer