caa a22 4500 467890412 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0249-5 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0249-5 Characterization of Citrus tristeza virus isolates in northern Iran [Elektronische Daten] [Ali Barzegar, Haleh Hashemi Sohi, Heshmatollah Rahimian] The biological and molecular properties of four Citrus tristeza virus (CTV) isolates isolated from infected Satsuma trees imported from Japan, and growing in citrus groves in northern Iran (Mahdasht orchards, Mazandaran Province), were investigated. CTV-infected samples were collected from sweet orange trees and grafted onto Alemow (Citrus macrophylla Wester) seedlings. On indicator plants, these isolates produced various symptoms including vein clearing and stem pitting on Mexican lime, Alemow, and Citrus hystrix, and yellowing and stunting on sour orange and grapefruit seedlings. Citrus samples were also surveyed for CTV using serological tests. The coat protein (CP) gene of these isolates was amplified using specific primers, yielding an amplicon of 672 bp for all isolates. Sequence analysis showed 98%-99% sequence homology of Iranian isolates with the Californian CTV severe stem-pitting isolate SY568 and 97%-98% homology with the Japanese seedling yellows isolate NUagA. The Iranian isolates were compared by restriction fragment length polymorphism (RFLP) analysis of the CP amplicon for further classification. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Citrus tristeza virus nationallicence Coat protein gene nationallicence Iranian isolates nationallicence Nucleotide sequencing nationallicence RFLP nationallicence Biological properties nationallicence Barzegar Ali Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran aut Sohi Haleh Hashemi Plant Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, 15 Shahid Shafiee Alley, Ghods Street, Enghelab Avenue, Tehran, Iran aut Rahimian Heshmatollah College of Agriculture, Mazandaran University, Khazarabad Street, Sari, Iran aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 46-51 1345-2630 72:1<46 2006 72 10327 https://doi.org/10.1007/s10327-005-0249-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0249-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barzegar Ali Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran aut NATIONALLICENCE 700 1- Sohi Haleh Hashemi Plant Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, 15 Shahid Shafiee Alley, Ghods Street, Enghelab Avenue, Tehran, Iran aut NATIONALLICENCE 700 1- Rahimian Heshmatollah College of Agriculture, Mazandaran University, Khazarabad Street, Sari, Iran aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 46-51 1345-2630 72:1<46 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer