caa a22 4500 467890439 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0243-y doi (NATIONALLICENCE)springer-10.1007/s10327-005-0243-y An antagonistic rhizoplane bacterium Pseudomonas sp. strain EC-S101 physiologically stresses a spinach root rot pathogen Aphanomyces cochlioides [Elektronische Daten] [Abhinandan Deora, Yasuyuki Hashidoko, Md. Tofazzal Islam, Yuriko Aoyama, Toshiaki Ito, Satoshi Tahara] We observed that an antagonistic rhizoplane bacterium Pseudomonas sp. strain EC-S101 induces excessive lateral and apical branching in the hyphae of a root rot phytopathogen Aphanomyces cochlioides AC-5 resulting in radial growth inhibition of hyphae in a dual culture assay. Confocal laser scanning microscopic observations using fluorescent stains indicated an increased quantity of nuclei and lipid bodies in the affected hyphae during the early stage (less affected hyphae) at day 3 of interaction. At a more advanced stage (severely affected hyphae) at day 3, nuclei became smaller and round-shaped compared with the oval shape in AC-5 control hyphae. After 7 days, nuclei disintegrated, and the nuclear materials were released into the disorganized cytoplasm. With transmission electron microscopy at 5 days of interaction, we found that the cell walls of AC-5 hyphae were considerably thicker than those of the control. Enlarged vacuoles, lipid bodies sunk into vacuoles, and vacuoles filled with electron-dense material, followed by an invagination of the AC-5 hyphal cell wall, were commonly observed. Nonmembranous electron-transparent inclusion bodies irregular in size were often distributed in the affected hyphae. By integrating our observations, we conclude that antagonistic effects evoked by strain EC-S101 resulted in the death of AC-5 hyphae, which might contribute to the suppression of A. cochlioides AC-5-linked damping-off disease in its host plants. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Antagonistic rhizobacteria nationallicence Anti-Peronosporomycetes nationallicence Confocal laser scanning microscopic observation nationallicence Excessive branching nationallicence Pseudomonas jessenii nationallicence Deora Abhinandan Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Hashidoko Yasuyuki Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Islam Md. Tofazzal Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Aoyama Yuriko Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Ito Toshiaki Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Tahara Satoshi Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 57-64 1345-2630 72:1<57 2006 72 10327 https://doi.org/10.1007/s10327-005-0243-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0243-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deora Abhinandan Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 700 1- Hashidoko Yasuyuki Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 700 1- Islam Md. Tofazzal Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 700 1- Aoyama Yuriko Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 700 1- Ito Toshiaki Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 700 1- Tahara Satoshi Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Kita-ku, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 57-64 1345-2630 72:1<57 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer