caa a22 4500 467890447 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0247-7 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0247-7 Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea [Elektronische Daten] [Michiyo Oshima, Shinpei Banno, Kiyotsugu Okada, Taeko Takeuchi, Makoto Kimura, Akihiko Ichiishi, Isamu Yamaguchi, Makoto Fujimura] Previously, we cloned a putative osmosensing histidine kinase gene (BcOS1) and revealed that a single amino acid substitution, isoleucine to serine at codon 365, conferred dicarboximide resistance in field isolates of Botrytis cinerea. This point mutation (type I) occurred within the restriction enzyme TaqI site of the wild-type BcOS1 gene. Thus, a procedure was developed for detecting the type I mutation of the BcOS1 gene using a polymerase chain reaction (PCR) in combination with restriction fragment-length polymorphism (RFLP). Diagnosis by PCR-RFLP was conducted on the 105 isolates isolated from 26 fields in Japan. All dicarboximide-sensitive isolates (49 isolates) had the wild-type BcOS1 gene, and the 43 isolates with the type I mutation were resistant to dicarboximides without exception. These data indicate that dicarboximide-resistant isolates with type I mutation are widespread throughout Japan. However, other types of dicarboximide resistance were detected among isolates from Osaka; among the 24 resistant isolates from Osaka, 12 had the BcOS1 gene without the type I mutation. BcOS1 gene sequencing of these resistant isolates classified them into two groups, type II and type III. The type II isolates have three amino acid substitutions within BcOS1p (368Val to Phe, 369Gln to His, and 447Thr to Ser). The type III isolates have two amino acid substitutions within BcOS1p (369Gln to Pro and 373Asn to Ser). These amino acid changes are located on the amino acid repeat domain in BcOS1p. The three types of resistant isolates were all moderately resistant to dicarboximides without significant osmotic sensitivity, and their pathogenicity on cucumber leaves was also very similar to that of the wild-type isolate. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Fungicide resistance nationallicence Two-component signal transduction nationallicence Botryotinia fuckeliana nationallicence Oshima Michiyo Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut Banno Shinpei Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut Okada Kiyotsugu Agricultural, Food and Environmental Sciences Research Center of Osaka Prefecture, Osaka, Japan aut Takeuchi Taeko Chiba Prefectural Agriculture Research Center, Chiba, Japan aut Kimura Makoto Plant Science Center, RIKEN, Yokohama, Japan aut Ichiishi Akihiko Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut Yamaguchi Isamu Plant Science Center, RIKEN, Yokohama, Japan aut Fujimura Makoto Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 65-73 1345-2630 72:1<65 2006 72 10327 https://doi.org/10.1007/s10327-005-0247-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0247-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oshima Michiyo Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut NATIONALLICENCE 700 1- Banno Shinpei Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut NATIONALLICENCE 700 1- Okada Kiyotsugu Agricultural, Food and Environmental Sciences Research Center of Osaka Prefecture, Osaka, Japan aut NATIONALLICENCE 700 1- Takeuchi Taeko Chiba Prefectural Agriculture Research Center, Chiba, Japan aut NATIONALLICENCE 700 1- Kimura Makoto Plant Science Center, RIKEN, Yokohama, Japan aut NATIONALLICENCE 700 1- Ichiishi Akihiko Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut NATIONALLICENCE 700 1- Yamaguchi Isamu Plant Science Center, RIKEN, Yokohama, Japan aut NATIONALLICENCE 700 1- Fujimura Makoto Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, 374-0193, Gunma, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 65-73 1345-2630 72:1<65 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer