caa a22 4500 467890455 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0242-z doi (NATIONALLICENCE)springer-10.1007/s10327-005-0242-z Erwinia amylovora can pass through the abscission layer of fruit-bearing twigs and invade apple fruit during fruit maturation [Elektronische Daten] [Koji Azegami, Takanori Tsukamoto, Takayuki Matsuura, Yasuhiro Inoue, Hiroshi Uematsu, Tatsuji Ohara, Akifumi Mizuno, Kouji Yoshida, Hideo Bessho, Shigeyoshi Sato, Shigeru Kimura, Masao Goto] Invasion of apple fruit by Erwinia amylovora from fruit-bearing twigs through the abscission layer at fruit maturation was examined. Erwinia amylovora (ca. 105 cfu) tagged with bioluminescence genes from Vibrio fischeri was deposited in artificial wounds on fruit-bearing twigs of apple trees grown in a containment greenhouse on September 22, 27, or October 5, 2004. On October 22, 176 apples were harvested and cut horizontally in half. The upper halves were stamped on plates of selective medium, and the lower halves were flooded with iodine solution to assess maturity. All fruit were symptomless and fully mature. The pathogen was recovered from 19 (10.8%) apples. The result showed that if at least ca. 105 cfu of E. amylovora are present in fruit-bearing twigs at the time of fruit maturation, the bacteria can pass through the abscission layer into the fruit, even though the mature fruit lack symptoms. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Apple nationallicence Fire blight nationallicence Erwinia amylovora nationallicence Abscission layer nationallicence Invasion nationallicence Dissemination nationallicence Azegami Koji National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Tsukamoto Takanori Yokohama Plant Protection Station, Yokohama, Japan aut Matsuura Takayuki National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Inoue Yasuhiro National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Uematsu Hiroshi Yokohama Plant Protection Station, Yokohama, Japan aut Ohara Tatsuji Yokohama Plant Protection Station, Yokohama, Japan aut Mizuno Akifumi Yokohama Plant Protection Station, Yokohama, Japan aut Yoshida Kouji National Institute of Fruit Tree Science, Tsukuba, Japan aut Bessho Hideo National Institute of Fruit Tree Science, Morioka, Japan aut Sato Shigeyoshi Yokohama Plant Protection Station, Yokohama, Japan aut Kimura Shigeru Yokohama Plant Protection Station, Yokohama, Japan aut Goto Masao Shizuoka University, Shizuoka, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 43-45 1345-2630 72:1<43 2006 72 10327 https://doi.org/10.1007/s10327-005-0242-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0242-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Azegami Koji National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Tsukamoto Takanori Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Matsuura Takayuki National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Inoue Yasuhiro National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Uematsu Hiroshi Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Ohara Tatsuji Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Mizuno Akifumi Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Yoshida Kouji National Institute of Fruit Tree Science, Tsukuba, Japan aut NATIONALLICENCE 700 1- Bessho Hideo National Institute of Fruit Tree Science, Morioka, Japan aut NATIONALLICENCE 700 1- Sato Shigeyoshi Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Kimura Shigeru Yokohama Plant Protection Station, Yokohama, Japan aut NATIONALLICENCE 700 1- Goto Masao Shizuoka University, Shizuoka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 43-45 1345-2630 72:1<43 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer