caa a22 4500 46789048X CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0244-x doi (NATIONALLICENCE)springer-10.1007/s10327-005-0244-x Bioluminescence reporter assay system to monitor Arabidopsis MPK3 gene expression in response to infection by Botrytis cinerea [Elektronische Daten] [Tsuneyuki Tanaka, Sachiko Ono, Yuriko Watakabe, Kazuyuki Hiratsuka] The Arabidopsis MPK3 gene product participates in disease resistance mediated by the MAP kinase cascade. The expression of the MPK3 gene is induced by pathogen inoculation and treatment with chemicals such as salicylic acid (SA) and methyl jasmonate (JA), but the detailed expression pattern of the MPK3 gene has been largely unknown. To investigate MPK3 gene expression in response to disease stress, we fused the MPK3 promoter to the firefly luciferase gene to create a real-time monitoring system for regulated gene expression in planta. The results of an in vivo reporter assay using transgenic Arabidopsis plants harboring MPK3::Fluc showed that the MPK3 promoter activity was induced by treatment with chemicals such as SA and benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH), that induce defense gene expression. Inoculation with the fungal pathogen Botrytis cinerea resulted in systemic induction of MPK3::Fluc. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Arabidopsis nationallicence Luciferase assay nationallicence Botrytis cinerea nationallicence MPK3 nationallicence Promoter nationallicence Tanaka Tsuneyuki Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut Ono Sachiko Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut Watakabe Yuriko Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan aut Hiratsuka Kazuyuki Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 1-5 1345-2630 72:1<1 2006 72 10327 https://doi.org/10.1007/s10327-005-0244-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0244-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Tsuneyuki Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Ono Sachiko Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Watakabe Yuriko Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan aut NATIONALLICENCE 700 1- Hiratsuka Kazuyuki Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, 240-8501, Yokohama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 1-5 1345-2630 72:1<1 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer