caa a22 4500 467890498 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0240-1 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0240-1 The hrpZ and hrpA genes are variable, and useful for grouping Pseudomonas syringae bacteria [Elektronische Daten] [Yasuhiro Inoue, Yuichi Takikawa] The hrpS to hrpB regions from strains of Pseudomonas syringae were amplified by polymerase chain reaction (PCR) and the DNA sequence determined. The order of hrpS, hrpA, hrpZ, and hrpB was consistent among P. syringae strains. The sequence of hrpS was highly conserved. In a cluster analysis with the hrpS sequence, P. syringae strains were divided into four groups (I, II, III, and IV) and one undetermined strain, in agreement with previous studies. In contrast, the hrpZ sequences contained insertions, deletions, and base substitutions followed by changes in amino acids. Based on cluster analysis of hrpA, hrpZ, and hrpB, P. syringae strains could be divided into five groups. One of the four groups (group I) in the cluster analysis of hrpS could be further divided into two subgroups (groups IA and IB). Groups II, III, and IV were the same in the two analyses. Group-specific primers were designed, based on the DNA sequences of hrpZ, that could differentiate the groups of P. syringae strains. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Pseudomonas syringae nationallicence Phylogenetic analysis nationallicence hrpZ nationallicence Group-specific primers nationallicence Inoue Yasuhiro Department of Plant Pathology, National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 26-33 1345-2630 72:1<26 2006 72 10327 https://doi.org/10.1007/s10327-005-0240-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0240-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inoue Yasuhiro Department of Plant Pathology, National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 26-33 1345-2630 72:1<26 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer