caa a22 4500 467890528 CHVBK 20180406152747.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s10327-005-0246-8 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0246-8 Flagella-mediated motility is required for biofilm formation by Erwinia carotovora subsp. carotovora [Elektronische Daten] [Md Mijan Hossain, Shinji Tsuyumu] To elucidate the role of flagella in biofilm formation by Erwinia carotovora subsp. carotovora EC1, we used a nonflagellate, nonmotile mutant (ΔfliC) and a flagellate, nonmotile mutant (ΔmotA). A biofilm-inducing medium, which contains the yeast peptone (YP) medium plus the salts of M-63 minimal medium, supported biofilm formation to a greater extent than either the YP or Luria Bertani (LB) medium alone. We demonstrated that both the ΔfliC and ΔmotA mutants greatly reduced their ability to form a biofilm on the surface of the wells of polyvinyl chloride (PVC) microtiter plates. The inability of both mutants to form biofilm on the PVC surface was further confirmed with phase-contrast microscopy. Both aflagellate (ΔfliC) and flagellate (ΔmotA) nonmotile mutants were equally defective in attachment to the PVC surface. The treatment of bacteria with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), which inhibits the motility of this organism, reduced greatly the biofilm formation. Based on these results, flagella-mediated motility may play an important role in biofilm formation of E. carotovora subsp. carotovora EC1. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Erwinia carotovora subsp. carotovora EC1 nationallicence Biofilm formation nationallicence Motility nationallicence Carbonyl cyanide m -chlorophenylhydrazone nationallicence Δ fliC nationallicence Δ motA nationallicence Hossain Md Mijan Faculty of Agriculture, Shizuoka University, Gifu United Graduate School of Agriculture, Shizuoka, Japan aut Tsuyumu Shinji Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan aut Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 34-39 1345-2630 72:1<34 2006 72 10327 https://doi.org/10.1007/s10327-005-0246-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0246-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hossain Md Mijan Faculty of Agriculture, Shizuoka University, Gifu United Graduate School of Agriculture, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tsuyumu Shinji Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/1(2006-02-01), 34-39 1345-2630 72:1<34 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer