caa a22 4500 467890544 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10327-006-0300-1 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0300-1 A simple method for a mini-preparation of fungal DNA [Elektronische Daten] [Ken-ichiro Saitoh, Kana Togashi, Tsutomu Arie, Tohru Teraoka] A simple method was established to prepare DNA from fungal mycelia cultured on an agar plate. The fungi tested successfully with this method contained Zygomycetes, Ascomycetes, Basidiomycetes, and Oomycetes. This method did not require any time-consuming steps to crush or digest mycelia or fractionation in a phenol-chloroform mixture. The DNA was easily extracted by immersing and dispersing the mycelial plugs in a specific buffer (200 mM Tris-HCl, 50 mM ethylenediaminetetraacetic acid, 200 mM NaCl, 1% n-lauroylsarcosine, pH 8.0), then concentrated by ethanol precipitation. The total time to complete the whole procedure was less than 1 h. The quality and quantity were sufficient for polymerase chain reaction amplification and Southern blot analysis. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Fungal DNA extraction nationallicence Zygomycetes nationallicence Ascomycetes nationallicence Basidiomycetes nationallicence Oomycetes nationallicence Saitoh Ken-ichiro Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut Togashi Kana Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut Arie Tsutomu Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut Teraoka Tohru Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 348-350 1345-2630 72:6<348 2006 72 10327 https://doi.org/10.1007/s10327-006-0300-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0300-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saitoh Ken-ichiro Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut NATIONALLICENCE 700 1- Togashi Kana Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut NATIONALLICENCE 700 1- Arie Tsutomu Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut NATIONALLICENCE 700 1- Teraoka Tohru Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 348-350 1345-2630 72:6<348 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer