caa a22 4500 467890579 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10327-006-0307-7 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0307-7 Molecular identification of some African strains of Ralstonia solanacearum from eucalypt and potato [Elektronische Daten] [Joanne Fouché-Weich, Stéphane Poussier, Danielle Trigalet-Demery, Dave Berger, Teresa Coutinho] Ralstonia solanacearum is a known bacterial pathogen of eucalypt and potato plants in Africa. A survey was undertaken to detect this pathogen in eucalypt plantations in South Africa, the Democratic Republic of Congo, and Uganda. Numerous bacterial strains were isolated from trees with symptoms typical of bacterial wilt, but only seven were positively identified as R. solanacearum. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, based on the hrp (hypersensitive response and pathogenicity) gene region was used to determine and group the biovars of these R. solanacearum strains. The eucalypt isolates and one potato isolate formed a biovar 3 cluster, whereas the two other potato isolates formed a cluster that corresponded to biovar 2. Amplified fragment length polymorphism (AFLP) analysis confirmed these clusters. Therefore, PCR-RFLP can be used as a reliable diagnostic technique to enable researchers to rapidly identify the pathogen. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Ralstonia solanacearum nationallicence Bacterial wilt nationallicence Diagnostics nationallicence PCR-RFLP nationallicence AFLP nationallicence Fouché-Weich Joanne Department of Botany, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, 0002, Pretoria, South Africa aut Poussier Stéphane Département des Sciences Biologiques, Unité de Protection des Plantes, Institut National d'Horticulture (INH), Angers, France aut Trigalet-Demery Danielle Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326, Castanet-Tolosan Cedex, France aut Berger Dave Department of Botany, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, 0002, Pretoria, South Africa aut Coutinho Teresa Department of Microbiology and Plant Pathology, FABI, University of Pretoria, Pretoria, South Africa aut Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 369-373 1345-2630 72:6<369 2006 72 10327 https://doi.org/10.1007/s10327-006-0307-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0307-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fouché-Weich Joanne Department of Botany, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, 0002, Pretoria, South Africa aut NATIONALLICENCE 700 1- Poussier Stéphane Département des Sciences Biologiques, Unité de Protection des Plantes, Institut National d'Horticulture (INH), Angers, France aut NATIONALLICENCE 700 1- Trigalet-Demery Danielle Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Berger Dave Department of Botany, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, 0002, Pretoria, South Africa aut NATIONALLICENCE 700 1- Coutinho Teresa Department of Microbiology and Plant Pathology, FABI, University of Pretoria, Pretoria, South Africa aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 369-373 1345-2630 72:6<369 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer