caa a22 4500 467890609 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10327-006-0310-z doi (NATIONALLICENCE)springer-10.1007/s10327-006-0310-z Identification of potential virulence genes in Erwinia chrysanthemi 3937: transposon insertion into plant-upregulated genes [Elektronische Daten] [Yasushi Okinaka, Nicole T. Perna, Shihui Yang, Noel T. Keen, Ching-Hong Yang] Erwinia chrysanthemi 3937 is a soft-rotting plant pathogen in Enterobacteriaceae. It attacks a wide range of plant host species. Previously, we identified dozens of E. chrysanthemi 3937 genes induced during plant infection by microarray differential display. Here, we have mutated plant-upregulated and putatively plant-upregulated genes in E. chrysanthemi 3937 using a transposon insertion method. Of 57 mutants produced, 8 were significantly reduced in maceration in African violet leaves. These 8 E. chrysanthemi genes are similar to Escherichia coli purU (formyltetrahydrofolate deformylase; ASAP20623) and wcaJ (undecaprenylphosphate glucosephosphotransferase; ASAP18556), Bacillus subtilis dltA (d-alanine-d-alanyl carrier protein ligase; ASAP19406), Pseudomonas syringae PSPTO2912 (ABC transporter, periplasmic glutamine-binding protein; ASAP15639), Pseudomonas aeruginosa pheC (cyclohexadienyl dehydratase; ASAP19773), P. syringae syrE (peptide synthase; ASAP19989), Vibrio vulnificus VV12303 (unknown protein; ASAP18555), and Yersinia pestis speD (S-adenosylmethionine decarboxylase; ASAP20536). In some of the genes, possible roles in virulence could be postulated based on the functions of their homologues. This work demonstrated that a low proportion of pathogenicity-related genes were among the plant-upregulated genes of E. chrysanthemi 3937. This study and further dissection of these putative virulence genes should lead to new insights into infection mechanisms in pathogens. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Erwinia chrysanthemi nationallicence Pectobacterium chrysanthemi nationallicence Transposon insertion nationallicence Identification of virulence genes nationallicence Plant-upregulated genes nationallicence Okinaka Yasushi Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan aut Perna Nicole T. Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, WI, USA aut Yang Shihui Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, Milwaukee, WI, USA aut Keen Noel T. Department of Plant Pathology, University of California, Riverside, CA, USA aut Yang Ching-Hong Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, Milwaukee, WI, USA aut Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 360-368 1345-2630 72:6<360 2006 72 10327 https://doi.org/10.1007/s10327-006-0310-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0310-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okinaka Yasushi Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Perna Nicole T. Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, WI, USA aut NATIONALLICENCE 700 1- Yang Shihui Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Keen Noel T. Department of Plant Pathology, University of California, Riverside, CA, USA aut NATIONALLICENCE 700 1- Yang Ching-Hong Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, Milwaukee, WI, USA aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 360-368 1345-2630 72:6<360 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer