caa a22 4500 467890633 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10327-006-0306-8 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0306-8 Quantification of DNA of citrus huanglongbing pathogen in diseased leaves using competitive PCR [Elektronische Daten] [Kunimasa Kawabe, Nguyen Thi Ngoc Truc, Bui Thi Ngoc Lan, Le Thi Thu Hong, Masatoshi Onuki] A quantification system for huanglongbing pathogen using a competitive polymerase chain reaction method and image-analyzing software were developed to obtain precise results. Significant differences in the quantity of pathogen were thus determined in leaves of two citrus cultivars commonly cultivated in southern Vietnam. Less pathogen-related DNA was detected from the tissue of citrus cultivars that are believed to be more tolerant than susceptible cultivars. The quantification system will be used in studies on pathogen proliferation and movement inside citrus tissue. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Huanglongbing nationallicence Citrus greening nationallicence Candidatus Liberibacter asiaticus nationallicence Competitive PCR nationallicence Quantification nationallicence Kawabe Kunimasa Japan International Research Center for Agriculture Science (JIRCAS), Tropical Agriculture Research Front, 907-0002, Okinawa, Japan aut Truc Nguyen Thi Ngoc Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut Lan Bui Thi Ngoc Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut Hong Le Thi Thu Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut Onuki Masatoshi Japan International Research Center for Agriculture Science (JIRCAS), Tropical Agriculture Research Front, 907-0002, Okinawa, Japan aut Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 355-359 1345-2630 72:6<355 2006 72 10327 https://doi.org/10.1007/s10327-006-0306-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0306-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawabe Kunimasa Japan International Research Center for Agriculture Science (JIRCAS), Tropical Agriculture Research Front, 907-0002, Okinawa, Japan aut NATIONALLICENCE 700 1- Truc Nguyen Thi Ngoc Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut NATIONALLICENCE 700 1- Lan Bui Thi Ngoc Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut NATIONALLICENCE 700 1- Hong Le Thi Thu Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam aut NATIONALLICENCE 700 1- Onuki Masatoshi Japan International Research Center for Agriculture Science (JIRCAS), Tropical Agriculture Research Front, 907-0002, Okinawa, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 355-359 1345-2630 72:6<355 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer