caa a22 4500 467890692 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s10327-006-0303-y doi (NATIONALLICENCE)springer-10.1007/s10327-006-0303-y Identification of cachexia-inducible Hop stunt viroid variants in citrus orchards in Japan using biological indexing and improved reverse transcription polymerase chain reaction [Elektronische Daten] [Takao Ito, Takane Furuta, Tsutae Ito, Masahiro Isaka, Yoichi Ide, Junko Kaneyoshi] The distribution of Hop stunt viroid (HSVd) variants related to cachexia disease in Japan was investigated using reverse transcription polymerase chain reaction (RT-PCR) to specifically detect the five nucleotide differences in the "cachexia motif.” With RT-PCR, HSVd variants having the cachexia motif were detected in samples from 10 of 43 citrus trees in experimental orchards and from only 3 of 530 in commercial orchards. Although the trees were symptomless cachexia carriers, biological indexing using "Parson's Special” mandarin (Citrus reticulata Blanco) verified the presence of the cachexia agent. RT-PCR with the primer pair CCV-M1 and CCV-P1 will be useful in preventing the spread of cachexia HSVd variants. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Biological indexing nationallicence Cachexia nationallicence Citrus nationallicence Hop stunt viroid nationallicence RT-PCR nationallicence Xyloporosis nationallicence Ito Takao Department of Citrus Research, National Institute of Fruit Tree Science, 859-2501, Nagasaki, Kuchinotsu, Japan aut Furuta Takane Biotechnological Research Division, Hiroshima Prefectural Agriculture Research Center, Hiroshima, Japan aut Ito Tsutae Department of Apple Research, National Institute of Fruit Tree Science, Iwate, Japan aut Isaka Masahiro Yamato Post-entry Quarantine Center, Yokohama Plant Protection Station, Kanagawa, Japan aut Ide Yoichi Saga Fruit Tree Experiment Station, Saga, Japan aut Kaneyoshi Junko Biotechnological Research Division, Hiroshima Prefectural Agriculture Research Center, Hiroshima, Japan aut Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 378-382 1345-2630 72:6<378 2006 72 10327 https://doi.org/10.1007/s10327-006-0303-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0303-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Takao Department of Citrus Research, National Institute of Fruit Tree Science, 859-2501, Nagasaki, Kuchinotsu, Japan aut NATIONALLICENCE 700 1- Furuta Takane Biotechnological Research Division, Hiroshima Prefectural Agriculture Research Center, Hiroshima, Japan aut NATIONALLICENCE 700 1- Ito Tsutae Department of Apple Research, National Institute of Fruit Tree Science, Iwate, Japan aut NATIONALLICENCE 700 1- Isaka Masahiro Yamato Post-entry Quarantine Center, Yokohama Plant Protection Station, Kanagawa, Japan aut NATIONALLICENCE 700 1- Ide Yoichi Saga Fruit Tree Experiment Station, Saga, Japan aut NATIONALLICENCE 700 1- Kaneyoshi Junko Biotechnological Research Division, Hiroshima Prefectural Agriculture Research Center, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/6(2006-12-01), 378-382 1345-2630 72:6<378 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer