caa a22 4500 467890749 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0267-3 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0267-3 Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms [Elektronische Daten] [Hirotaka Takahashi, Kazuhiro Toyoda, Yuzo Hirakawa, Kunihiko Morishita, Toshiaki Kato, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi] Apyrases (NTPases) are associated with both compatible and incompatible interactions between plants and microorganisms. Previously we reported that the ATPase activities of cell-wall-bound apyrases of several leguminous plants, such as pea, cowpea, soybean, and kidney bean, were enhanced by a glycoprotein elicitor and were inhibited in a species-specific manner by mucin-type glycopeptide suppressors secreted from a pea pathogenic fungus, Mycosphaerella pinodes. In this study, we isolated two apyrase genes, VsNTPase1 and VsNTPase2, from a cDNA library of Vigna sinensis Endl. cv. Sanjakusasage. Based on phylogenetic analysis, VsNTPase1 may belong to a group that responds to environmental stimuli. In a transient assay using DNA bombardment, a fusion protein of green fluorescent protein (GFP) and the N-terminal putative signal sequence of VsNTPase1 was distributed in the nucleus, cytoplasm (cytoskeletal structure), and cell wall. On the other hand, a fusion protein of GFP and the N-terminal putative VsNTPase2-signal sequence was localized in the cytoplasm, especially in small particles (perhaps mitochondria). A recombinant VsNTPase1 expressed in Spodoptera frugiperda 21 cells responded directly to signal molecules from several phytopathogenic microorganisms. Here, we discuss the role of apyrases in recognizing and responding to exogenous signals. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Apyrase nationallicence Elicitor nationallicence Mycosphaerella pinodes nationallicence Suppressor nationallicence Vigna sinensis Endl., cv. Sanjakusasage nationallicence Takahashi Hirotaka Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Hirakawa Yuzo Nishikawa Rubber Co. Ltd., Hiroshima, Japan aut Morishita Kunihiko Wako Pure Chemical Industries, Ltd., Hiroshima, Japan aut Kato Toshiaki Advanced Materials and Technology Research Laboratory, Nippon Steel Co. Ltd., Chiba, Japan aut Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 143-151 1345-2630 72:3<143 2006 72 10327 https://doi.org/10.1007/s10327-005-0267-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0267-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Hirotaka Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Hirakawa Yuzo Nishikawa Rubber Co. Ltd., Hiroshima, Japan aut NATIONALLICENCE 700 1- Morishita Kunihiko Wako Pure Chemical Industries, Ltd., Hiroshima, Japan aut NATIONALLICENCE 700 1- Kato Toshiaki Advanced Materials and Technology Research Laboratory, Nippon Steel Co. Ltd., Chiba, Japan aut NATIONALLICENCE 700 1- Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 143-151 1345-2630 72:3<143 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer