caa a22 4500 467890757 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0269-1 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0269-1 Fluorescent staining of resting spores of Polymyxa betae as a fungal vector of rhizomania disease of sugar beet in soil [Elektronische Daten] [Mitsuru Sayama, Yoji Momota, Shigehito Takenaka] When the resting spores of Polymyxa betae were pretreated with 2% sodium dodecyl sulfate (SDS) and then stained with various fluorochromes including 3,3′-dihexyloxacarbocyanine iodide [DiOC6(3)], calcofluor, and a fluorescein isothiocyanate (FITC)-conjugated lectin, such as wheat germ lectin (WGA) or caster bean lectin, most spores fluoresced brightly. FITC-WGA mainly stained the cell surface, while DiOC6(3) stained the cytoplasm. After pretreatment with SDS and addition of FITC-WGA or DiOC6(3) to a soil suspension containing resting spores, the resting spores were distinguishable from the soil particles. This staining method is easy to use for the detection of resting spores in the soil. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Fluorochrome nationallicence Lectin nationallicence Polymyxa betae nationallicence Rhizomania nationallicence Sugar beet nationallicence Sayama Mitsuru Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, Japan aut Momota Yoji Department of Agro-Environment Sciences, National Agricultural Research Center for Hokkaido Region, Sapporo, Japan aut Takenaka Shigehito Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 190-192 1345-2630 72:3<190 2006 72 10327 https://doi.org/10.1007/s10327-005-0269-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0269-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sayama Mitsuru Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Momota Yoji Department of Agro-Environment Sciences, National Agricultural Research Center for Hokkaido Region, Sapporo, Japan aut NATIONALLICENCE 700 1- Takenaka Shigehito Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 190-192 1345-2630 72:3<190 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer