caa a22 4500 467890773 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0265-5 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0265-5 In vitro but not in planta encapsidation of Rice gall dwarf virus core particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants [Elektronische Daten] [Kyoji Hagiwara, Takahiko Higashi, Kazunari Takahashi, Naho Hara, Hideyuki Aoki, Naoyuki Miyazaki, Qing-Yu Wang, Yafeng Zhu, Osamu Yatou, Hiroshi Tanaka, Toshihiro Omura] Transencapsidation of the Rice gall dwarf virus (RGDV) inner core by the Rice dwarf virus (RDV) outer capsid P8 protein was examined in vitro and in planta. When RGDV core particles were incubated with an extract from RDV P8-transgenic rice leaf tissue, RDV P8 encapsidated the RGDV core particles to form double-shelled virus-like particles in vitro. In contrast, when RDV P8-transgenic rice plants were inoculated with RGDV, progeny RGDV particles contained RGDV P8 but RDV P8 was not detectable in the virions. No significant differences were found in acquisition by the vector insects and subsequent transmission rates between RGDV infecting nontransgenic rice plants and those infecting RDV P8-transgenic rice plants. These results indicate that mechanisms of and/or requirements for interactions between P8 and the inner core particles of phytoreoviruses differ between in vitro and in planta. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Encapsidation nationallicence Outer capsid protein nationallicence Rice dwarf virus nationallicence Rice gall dwarf virus nationallicence Hagiwara Kyoji National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Higashi Takahiko National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Takahashi Kazunari National Institute of Agrobiological Sciences, Tsukuba, Japan aut Hara Naho National Institute of Agrobiological Sciences, Tsukuba, Japan aut Aoki Hideyuki Hokuriku Research Center, NARC, Niigata, Japan aut Miyazaki Naoyuki National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Wang Qing-Yu Hokuriku Research Center, NARC, Niigata, Japan aut Zhu Yafeng National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Yatou Osamu Hokuriku Research Center, NARC, Niigata, Japan aut Tanaka Hiroshi National Institute of Agrobiological Sciences, Tsukuba, Japan aut Omura Toshihiro National Agricultural Research Center, 305-8666, Tsukuba, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 186-189 1345-2630 72:3<186 2006 72 10327 https://doi.org/10.1007/s10327-005-0265-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0265-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hagiwara Kyoji National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Higashi Takahiko National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Takahashi Kazunari National Institute of Agrobiological Sciences, Tsukuba, Japan aut NATIONALLICENCE 700 1- Hara Naho National Institute of Agrobiological Sciences, Tsukuba, Japan aut NATIONALLICENCE 700 1- Aoki Hideyuki Hokuriku Research Center, NARC, Niigata, Japan aut NATIONALLICENCE 700 1- Miyazaki Naoyuki National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Wang Qing-Yu Hokuriku Research Center, NARC, Niigata, Japan aut NATIONALLICENCE 700 1- Zhu Yafeng National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Yatou Osamu Hokuriku Research Center, NARC, Niigata, Japan aut NATIONALLICENCE 700 1- Tanaka Hiroshi National Institute of Agrobiological Sciences, Tsukuba, Japan aut NATIONALLICENCE 700 1- Omura Toshihiro National Agricultural Research Center, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 186-189 1345-2630 72:3<186 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer