caa a22 4500 46789079X CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0261-9 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0261-9 Induction of defense responses in pea tissues by inorganic phosphate [Elektronische Daten] [Tomoharu Kawahara, Hiroko Namba, Kazuhiro Toyoda, Tomonari Kasai, Megumi Sugimoto, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi] When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3 h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Defense nationallicence Inorganic phosphate signaling pathway nationallicence Mycosphaerella pinodes nationallicence Extracellular peroxidase nationallicence Pisum sativum L nationallicence Kawahara Tomoharu Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Namba Hiroko Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Kasai Tomonari Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Sugimoto Megumi Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 129-136 1345-2630 72:3<129 2006 72 10327 https://doi.org/10.1007/s10327-005-0261-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0261-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawahara Tomoharu Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Namba Hiroko Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Kasai Tomonari Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Sugimoto Megumi Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 129-136 1345-2630 72:3<129 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer