caa a22 4500 467890811 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0271-7 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0271-7 Analysis of the gacS-gacA regulatory genes of spontaneous mutants of Pseudomonas fluorescens biocontrol strain PfG32R [Elektronische Daten] [W. Gusti Ngurah Alit-Susanta, Yuichi Takikawa] Pseudomonas fluorescens strain PfG32R, a potential biocontrol agent against soilborne pathogens, frequently loses its antifungal activity and ability to produce enzymes. To characterize genetically the instability of these bacterial functions, we analyzed gacS and gacA genes of PfG32R and three spontaneous mutants of PfG32R (NR1, NR9, and ASW6), which had lost their ability to produce proteases and antifungal activities. The gacS and gacA sequences of PfG32R had 77%-89% and 78%-87% homology, respectively, with several known gacS and gacA homologues. All three spontaneous mutants were subjected to complementation analysis. Introduction of clones containing an intact gacS of PfG32R and another P. fluorescens strain Pf-5 as well as strain tea632 of P. syringae pv. theae complemented all three mutants restoring protease and antifungal activities, indicating a mutation in gacS. In sequencing analysis, the mutants had a deletion or change in amino acids in the conserved sensor kinase domains of GacS. The three mutants maintained both their antibacterial activity against Ralstonia solanacearum and Clavibacter michiganensis ssp. michiganensis and siderophore production, indicating that they are not controlled by the GacS/GacA system. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Pseudomonas fluorescens PfG32R nationallicence Two-component regulatory system nationallicence gacS mutants nationallicence Protease nationallicence Antifungal activity nationallicence Antibacterial activity nationallicence Alit-Susanta W. Gusti Ngurah Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 159-167 1345-2630 72:3<159 2006 72 10327 https://doi.org/10.1007/s10327-005-0271-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0271-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alit-Susanta W. Gusti Ngurah Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 700 1- Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 159-167 1345-2630 72:3<159 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer