caa a22 4500 46789082X CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10327-005-0263-7 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0263-7 Phenotypic characterization of Pseudomonas fluorescens PfG32R and its spontaneous gacS mutants and biocontrol activity against bacterial wilt disease of tomato [Elektronische Daten] [W. Gusti Ngurah Alit-Susanta, Yuichi Takikawa] Pseudomonas fluorescens strain PfG32R actively suppresses the occurrence of bacterial wilt disease of tomato caused by Ralstonia solanacearum. To determine the characteristics affected by spontaneous mutations in the gacS gene, three spontaneous mutants NR1, NR9, and ASW6 were tested for production of several enzymes, antimicrobial activity, and biocontrol activity against bacterial wilt disease of tomato in a greenhouse. Production of biosurfactant and of enzymes such as Tween-80 lipase, gelatin protease, and lecithinase; swarming activity; and antifungal activities against Fusarium oxysporum f. sp. radicis-lycopersici and F. graminearum were inactivated by mutations in the gacS gene, indicating that they are under the regulation of the GacS/GacA two-component regulatory system. Levan production, swimming, antifungal activity against Magnaporthe grisea, and antibacterial activity against R. solanacearum and Erwinia carotovora ssp. carotovora were not controlled by the GacS/GacA system. Biocontrol activities of the three spontaneous mutants varied from strain to strain, indicating that the biocontrol activities of PfG32R are influenced not only by the GacS/GacA system but also by other complex factors, which may not be regulated by the system. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Pseudomonas fluorescens nationallicence gacS mutants nationallicence Bacterial wilt of tomato nationallicence Swarming nationallicence Biosurfactant nationallicence Antimicrobial activity nationallicence Alit-Susanta W. Gusti Ngurah Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 168-175 1345-2630 72:3<168 2006 72 10327 https://doi.org/10.1007/s10327-005-0263-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0263-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alit-Susanta W. Gusti Ngurah Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 700 1- Takikawa Yuichi Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/3(2006-06-01), 168-175 1345-2630 72:3<168 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer