caa a22 4500 467890838 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10327-006-0273-0 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0273-0 Black root rot of cucurbits caused by Phomopsis sclerotioides in Japan and phylogenetic grouping of the pathogen [Elektronische Daten] [Masahiro Shishido, Nanako Yoshida, Toshiyuki Usami, Tetsuo Shinozaki, Masanobu Kobayashi, Taeko Takeuchi] Although the causal agent of black root rot of Cucurbitaceae in Japan has been proposed as Phomopsis sclerotioides, the species identification of the pathogen has remained inconclusive because of a lack of spore formation. We confirmed that a Japanese isolate of Phomopsis sp. obtained from a diseased pumpkin root produced pycnidia containing α spores in sterilized bean pods. In phylogenetic analyses of rDNA-ITS regions, nine Japanese Phomopsis sp. isolates from melon, watermelon grafted onto bottle gourd, and pumpkin diagnosed with black root rot, formed a single clade with P. sclerotioides standard isolates. We identified the causal agent of the black root rot of melon, pumpkin, bottle gourd, and watermelon in Japan as P. sclerotioides and propose the Japanese name "Phomopsis-negusare-byo” for the disease. Patterns of random amplified polymorphic DNA (RAPD) of these Japanese isolates were also similar to those of P. sclerotioides, thus supporting the species identification. However, mycelial incompatibilities were found for many combinations among these P. sclerotioides isolates, suggesting some genotypic variations of this fungus in Japan at a level that the RAPD analyses cannot discriminate. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Phomopsis sclerotioides nationallicence Pycnospore nationallicence ITS region nationallicence Random amplified polymorphic DNA (RAPD) nationallicence Mycelial compatibility group (MCG) nationallicence Shishido Masahiro Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut Yoshida Nanako Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut Usami Toshiyuki Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut Shinozaki Tetsuo Japan Horticultural Production and Research Institute, Chiba, Matsudo, Japan aut Kobayashi Masanobu Kanagawa Prefectural Government Agriculture Promotion Division, Hiratsuka, Kanagawa, Japan aut Takeuchi Taeko Chiba Prefectural Agriculture Research Center, Chiba, Japan aut Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 220-227 1345-2630 72:4<220 2006 72 10327 https://doi.org/10.1007/s10327-006-0273-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0273-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shishido Masahiro Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut NATIONALLICENCE 700 1- Yoshida Nanako Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut NATIONALLICENCE 700 1- Usami Toshiyuki Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Chiba, Japan aut NATIONALLICENCE 700 1- Shinozaki Tetsuo Japan Horticultural Production and Research Institute, Chiba, Matsudo, Japan aut NATIONALLICENCE 700 1- Kobayashi Masanobu Kanagawa Prefectural Government Agriculture Promotion Division, Hiratsuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Takeuchi Taeko Chiba Prefectural Agriculture Research Center, Chiba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 220-227 1345-2630 72:4<220 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer