caa a22 4500 467890870 CHVBK 20180406152748.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10327-006-0277-9 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0277-9 DNA transposon fossils present on the conditionally dispensable chromosome controlling AF-toxin biosynthesis and pathogenicity of Alternaria alternata [Elektronische Daten] [Rieko Hatta, Akihisa Shinjo, Sheila Ruswandi, Keiji Kitani, Mikihiro Yamamoto, Kazuya Akimitsu, Takashi Tsuge] The strawberry pathotype of Alternaria alternata produces host-specific AF-toxin and causes Alternaria black spot of strawberry. Previously, we isolated cosmid clones pcAFT-1 and pcAFT-2 from strain NAF8 of the strawberry pathotype that contain AF-toxin biosynthetic genes, named AFT genes. In a molecular characterization here of pcAFT-1 and pcAFT-2, 11 AFT genes and five transposon-like sequences, named TLS-S1 to TLS-S5, were detected. The nucleotide sequences of TLS-S1 and TLS-S4 share high homology, and their putative products have similarity to transposases of the hAT family transposons. Thus, TLS-S1 and TLS-S4 were renamed TLS-S1-1 and TLS-S1-2, respectively. Amino acid sequences deduced from TLS-S2, TLS-S3, and TLS-S5 have similarity to transposases of the Fot1/Pogo family transposons, but they are significantly different. All five sequences have incomplete open reading frames (ORFs) for transposases owing to deletions, termination codons, and/or frameshifts, indicating that they are inactivated elements. Analysis of genomic distribution of these sequences revealed that they are specifically distributed on a 1.05-Mb chromosome of NAF8, which has been identified as a conditionally dispensable (CD) chromosome encoding AFT genes. The presence of three, four, and three copies of TLS-S1, TLS-S2, and TLS-S3, respectively, and a single copy of TLS-S5 on the CD chromosome were estimated by DNA gel blot analysis. The remaining copy of TLS-S1 and the three copies of TLS-S2 were isolated and identified to also encode incomplete ORFs. Thus, it appears that all copies of the transposon-like sequences identified are inactivated elements (fossils) unique to the CD chromosome in the genome of the strawberry pathotype. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Alternaria alternata nationallicence AF-toxin nationallicence Conditionally dispensable chromosome nationallicence Host-specific toxin nationallicence Class II transposon nationallicence Hatta Rieko Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut Shinjo Akihisa Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut Ruswandi Sheila Faculty of Agriculture, Okayama University, Okayama, Japan aut Kitani Keiji Faculty of Agriculture, Okayama University, Okayama, Japan aut Yamamoto Mikihiro Faculty of Agriculture, Okayama University, Okayama, Japan aut Akimitsu Kazuya Faculty of Agriculture, Kagawa University, Kagawa, Japan aut Tsuge Takashi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 210-219 1345-2630 72:4<210 2006 72 10327 https://doi.org/10.1007/s10327-006-0277-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0277-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatta Rieko Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut NATIONALLICENCE 700 1- Shinjo Akihisa Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut NATIONALLICENCE 700 1- Ruswandi Sheila Faculty of Agriculture, Okayama University, Okayama, Japan aut NATIONALLICENCE 700 1- Kitani Keiji Faculty of Agriculture, Okayama University, Okayama, Japan aut NATIONALLICENCE 700 1- Yamamoto Mikihiro Faculty of Agriculture, Okayama University, Okayama, Japan aut NATIONALLICENCE 700 1- Akimitsu Kazuya Faculty of Agriculture, Kagawa University, Kagawa, Japan aut NATIONALLICENCE 700 1- Tsuge Takashi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Chikusa, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 210-219 1345-2630 72:4<210 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer