caa a22 4500 467890897 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10327-006-0279-7 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0279-7 A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms [Elektronische Daten] [Akinori Kiba, Kazuhiro Toyoda, Kazuaki Yoshioka, Kami Tsujimura, Hirotaka Takahashi, Yuki Ichinose, Tadahiro Takeda, Toshiaki Kato, Tomonori Shiraishi] Apyrases (E.C.3.6.1.5; NTP-NDPases) are distributed in the cytosol, nuclei, cytoskeleton, and on the surface of plant cells. Some may play an important role in signal transduction from exogenous stimuli. We previously found a protein of ca. 55-kDa (CWP-55) in an ATPase-rich fraction from the pea cell wall bound to the elicitor and supprescins (suppressors of defense) from pea pathogen Mycosphaerella pinodes. We cloned the cDNA of CWP-55 that coincided with PsAPY1, one of two NTPase clones in a pea cDNA library. An analysis with a green fluorescent protein fusion protein indicated that PsAPY1 was distributed in the cell wall, nucleus, and cytoplasm. The recombinant PsAPY1 expressed in Escherichia coli had ATP-hydrolyzing activity responsive not only to the elicitor and supprescins from the pea pathogen but also to other elicitors such as a bacterial harpin, a yeast extract, and a synthetic glycopeptide. Biotinylated fungal signal molecules were bound to the recombinant PsAPY1 specifically. Resonant mirror detection confirmed such binding characteristics of PsAPY1. Based on these results, we discuss the role of cell-wall-bound NTPases in recognizing and responding to microorganisms on the cell wall surface. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Binding protein nationallicence Cell wall nationallicence Elicitor nationallicence NTPase nationallicence Receptor nationallicence Suppressor of defense nationallicence Kiba Akinori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Yoshioka Kazuaki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Tsujimura Kami Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Takahashi Hirotaka Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Takeda Tadahiro Kyoritsu College of Pharmacy, Tokyo, Japan aut Kato Toshiaki Nippon Steel Co. Ltd, Chiba, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 238-246 1345-2630 72:4<238 2006 72 10327 https://doi.org/10.1007/s10327-006-0279-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0279-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiba Akinori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Yoshioka Kazuaki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Tsujimura Kami Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Takahashi Hirotaka Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Takeda Tadahiro Kyoritsu College of Pharmacy, Tokyo, Japan aut NATIONALLICENCE 700 1- Kato Toshiaki Nippon Steel Co. Ltd, Chiba, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 238-246 1345-2630 72:4<238 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer