caa a22 4500 467890900 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10327-006-0278-8 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0278-8 A binding protein for fungal signal molecules in the cell wall of Pisum sativum [Elektronische Daten] [Akinori Kiba, Takako Ohgawara, Kazuhiro Toyoda, Miho Inoue-Ozaki, Tadahiro Takeda, Uppalapati Srinivasa Rao, Toshiaki Kato, Yuki Ichinose, Tomonori Shiraishi] In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous signal molecules. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Binding protein for fungal signals nationallicence Cell-wall-bound ATPase (NTPase) nationallicence Elicitor nationallicence Mycosphaerella pinodes nationallicence Pisum sativum L nationallicence Suppressor of defense nationallicence Kiba Akinori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Ohgawara Takako Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Inoue-Ozaki Miho School of Dentistry, Okayama University, Okayama, Japan aut Takeda Tadahiro Kyoritsu College of Pharmacy, Tokyo, Japan aut Rao Uppalapati Srinivasa Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Kato Toshiaki Nippon Steel Co. Ltd., Chiba, Japan aut Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 228-237 1345-2630 72:4<228 2006 72 10327 https://doi.org/10.1007/s10327-006-0278-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0278-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiba Akinori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 700 1- Ohgawara Takako Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 700 1- Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 700 1- Inoue-Ozaki Miho School of Dentistry, Okayama University, Okayama, Japan aut NATIONALLICENCE 700 1- Takeda Tadahiro Kyoritsu College of Pharmacy, Tokyo, Japan aut NATIONALLICENCE 700 1- Rao Uppalapati Srinivasa Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 700 1- Kato Toshiaki Nippon Steel Co. Ltd., Chiba, Japan aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, 700-8530, Okayama, Tsushima, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/4(2006-08-01), 228-237 1345-2630 72:4<228 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer