caa a22 4500 467890935 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10327-006-0287-7 doi (NATIONALLICENCE)springer-10.1007/s10327-006-0287-7 PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici [Elektronische Daten] [Yasushi Hirano, Tsutomu Arie] The pathogenic type (form and race) of Fusarium oxysporum, which generates wilt symptoms on tomato, was rapidly identified with a polymerase chain reaction (PCR)-based technique. We compared the partial nucleotide sequences of endo polygalacturonase (pg1) and exo polygalacturonase (pgx4) genes from isolates of F. oxysporum ff. sp. lycopersici (FOL) and radicis-lycopersici (FORL) from Japan and designed specific primer sets (uni, sp13, sp23, and sprl) based on the nucleotide differences that appeared among the pathogenic types. PCR with the uni primer set amplified a 670∼672-bp fragment from all isolates of FOL and FORL. With the sp13 primer set, an amplicon of 445 bp was obtained only from isolates of FOL race 1 and 3. With the sp23 primer set, a 518-bp fragment was obtained from isolates of FOL race 2 and 3. The sprl primer set yielded a 947-bp fragment from isolates of FORL, but not from FOL. A combination of amplifications with these primer sets effectively differentiated the pathogenic types of F. oxysporum in tomato. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Diagnosis nationallicence Forma specialis nationallicence Identification nationallicence Polygalacturonase nationallicence Nucleotide polymorphism nationallicence Tomato nationallicence Hirano Yasushi Saitama Prefectural Agriculture and Forestry Research Center, Saitama, Kuki, Japan aut Arie Tsutomu Tokyo University of Agriculture and Technology (TUAT), Saiwaicho, Fuchu, 183-8509, Tokyo, Japan aut Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 273-283 1345-2630 72:5<273 2006 72 10327 https://doi.org/10.1007/s10327-006-0287-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0287-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirano Yasushi Saitama Prefectural Agriculture and Forestry Research Center, Saitama, Kuki, Japan aut NATIONALLICENCE 700 1- Arie Tsutomu Tokyo University of Agriculture and Technology (TUAT), Saiwaicho, Fuchu, 183-8509, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 273-283 1345-2630 72:5<273 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer