caa a22 4500 467890943 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10327-006-0292-x doi (NATIONALLICENCE)springer-10.1007/s10327-006-0292-x An oligonucleotide probe for the detection of Erwinia herbicola and Erwinia ananas [Elektronische Daten] [Yoshihiro Nakanishi, Appolinaire Adandonon, Ikuko Okabe, Yuko T. Hoshino, Naoyuki Matsumoto] An oligonucleotide probe targeting the rRNA of Erwinia herbicola and Erwinia ananas was designed to detect their cells using fluorescence in situ hybridization. The Cy3-labeled probe hybridized strongly with these species but very weakly with nontarget species such as Erwinia mallotivora, Erwinia nigrifluens, Erwinia cypripedii, Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli. This technique visualized E. herbicola cells after inoculation of kumquat fruits. The probe is promising as a tool for studying population dynamics of E. herbicola and E. ananas. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Erwinia ananas nationallicence Erwinia herbicola nationallicence Fluorescence in situ hybridization (FISH) nationallicence Oligonucleotide probe nationallicence Pantoea nationallicence Nakanishi Yoshihiro Kagoshima Agricultural Experiment Station, Kagoshima, Japan aut Adandonon Appolinaire National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut Okabe Ikuko National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut Hoshino Yuko T. National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut Matsumoto Naoyuki National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 328-333 1345-2630 72:5<328 2006 72 10327 https://doi.org/10.1007/s10327-006-0292-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0292-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakanishi Yoshihiro Kagoshima Agricultural Experiment Station, Kagoshima, Japan aut NATIONALLICENCE 700 1- Adandonon Appolinaire National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut NATIONALLICENCE 700 1- Okabe Ikuko National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut NATIONALLICENCE 700 1- Hoshino Yuko T. National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut NATIONALLICENCE 700 1- Matsumoto Naoyuki National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 328-333 1345-2630 72:5<328 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer