caa a22 4500 467890951 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10327-006-0290-z doi (NATIONALLICENCE)springer-10.1007/s10327-006-0290-z DNA fingerprinting of Pyricularia grisea by rep-PCR using a single primer based on the terminal inverted repeat from either of the transposable elements Pot2 and MGR586 [Elektronische Daten] [Fumihiko Suzuki, Michiyoshi Arai, Junichiro Yamaguchi] We investigated the use of single primers complementary to sequences in the terminal inverted repeat (TIR) of either Pot2 or MGR586, transposable elements found in Pyricularia grisea, for DNA fingerprinting by repetitive-element-based polymerase chain reaction (rep-PCR). Under standard amplification conditions, rep-PCR with each single primer generated distinct fingerprint patterns among rice-infecting P. grisea isolates collected in Japan. With the Pot2-TIR primer, bands ranging in size from 0.2 to 8 kb and in number from 8 to 13 per isolate were amplified. Although fewer bands were amplified with the MGR586-TIR primer, this molecular technique should be more reliable to identify and classify P. grisea isolates by combining the data of fingerprint patterns from each TIR primer. In a cluster analysis based on DNA fingerprints from this rep-PCR with the Pot2-TIR primer, 10 reference isolates and 12 field isolates from Saga Prefecture in 2002 were separated into six clonal lineages. We also demonstrated that the 12 field isolates belonged to one clonal lineage. Thus, this rep-PCR method using the single primer Pot2-TIR will be useful for the analysis of the population structure of rice blast pathogens. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Pyricularia grisea nationallicence Rep-PCR nationallicence Pot 2 nationallicence MGR586 nationallicence Terminal inverted repeat nationallicence Clonal lineage nationallicence Suzuki Fumihiko National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, 861-1192, Kumamoto, Koshi, Japan aut Arai Michiyoshi National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, 861-1192, Kumamoto, Koshi, Japan aut Yamaguchi Junichiro Saga Agricultural Experiment Research Center, Saga, Japan aut Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 314-317 1345-2630 72:5<314 2006 72 10327 https://doi.org/10.1007/s10327-006-0290-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0290-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Fumihiko National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, 861-1192, Kumamoto, Koshi, Japan aut NATIONALLICENCE 700 1- Arai Michiyoshi National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, 861-1192, Kumamoto, Koshi, Japan aut NATIONALLICENCE 700 1- Yamaguchi Junichiro Saga Agricultural Experiment Research Center, Saga, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 314-317 1345-2630 72:5<314 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer