caa a22 4500 467891028 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10327-006-0283-y doi (NATIONALLICENCE)springer-10.1007/s10327-006-0283-y Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen [Elektronische Daten] [Tomonari Kasai, Tomoko Suzuki, Kozue Ono, Ken'ichi Ogawa, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Tomonori Shiraishi] We previously reported that the release of O2 − from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Apoplast nationallicence Cell wall nationallicence Cu/Zn superoxide dismutase (EC 1. 15. 1. 1) nationallicence Defense response nationallicence Mycosphaerella pinodes nationallicence Pisum sativum L nationallicence Kasai Tomonari Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Suzuki Tomoko Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Ono Kozue Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Ogawa Ken'ichi Research Institute for Biological Sciences, Okayama (RIBS Okayama), Japan aut Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 265-272 1345-2630 72:5<265 2006 72 10327 https://doi.org/10.1007/s10327-006-0283-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-006-0283-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kasai Tomonari Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Suzuki Tomoko Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ono Kozue Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ogawa Ken'ichi Research Institute for Biological Sciences, Okayama (RIBS Okayama), Japan aut NATIONALLICENCE 700 1- Inagaki Yoshishige Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Toyoda Kazuhiro Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology and Genetic Engineering, The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/5(2006-10-01), 265-272 1345-2630 72:5<265 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer