caa a22 4500 467891036 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10327-005-0260-x doi (NATIONALLICENCE)springer-10.1007/s10327-005-0260-x Incidence of thiophanate-methyl resistance in Cercospora kikuchii within a single lineage based on amplified fragment length polymorphisms in Japan [Elektronische Daten] [Iori Imazaki, Kouji Ishikawa, Nobuko Yasuda, Atsushi Miyasaka, Shinji Kawasaki, Shinzo Koizumi] We collected 247 isolates of Cercospora kikuchii from soybean seeds with typical purple stain symptoms from 15 prefectures in Japan. Of the 247 isolates, 93 were sensitive to thiophanate-methyl, a benzimidazole used to control this soybean disease; the remaining 154 were highly resistant to the fungicide. To examine genetic variability among the population of 247 isolates, we developed amplified fragment length polymorphism (AFLP) markers. An AFLP primer pair generated DNA fingerprint polymorphisms among the sample isolates, and with the unweighted pair-grouping method to cluster arithmetic means of the similarity coefficients among all pairs of the fingerprint patterns, the isolates were divided into four lineages (I to IV). Of the 247 isolates, 225 belonged to lineage I, including all isolates that were resistant to thiophanate-methyl. To determine whether the resistance of these isolates was related to mutations in the β-tubulin gene, we amplified partial nucleotide sequences of the gene from 29 representative isolates, including 12 that were resistant to thiophanate-methyl, by means of the polymerase chain reaction. The resistant isolates had identical nucleotide sequence with a one-step change at codon 198, in which the amino acid glutamic acid had been replaced by alanine. The evidence thus suggests that thiophanate-methyl resistance might have arisen in lineage I, the largest of the four lineages. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Thiophanate-methyl resistance nationallicence Cercospora kikuchii nationallicence Genetic structure nationallicence Imazaki Iori National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Ishikawa Kouji Niigata Agricultural Research Institute, Nagaoka, Japan aut Yasuda Nobuko National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Miyasaka Atsushi National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Kawasaki Shinji National Institute of Agrobiological Sciences, Tsukuba, Japan aut Koizumi Shinzo National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut Journal of General Plant Pathology Springer-Verlag 72/2(2006-04-01), 77-84 1345-2630 72:2<77 2006 72 10327 https://doi.org/10.1007/s10327-005-0260-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0260-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Imazaki Iori National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Ishikawa Kouji Niigata Agricultural Research Institute, Nagaoka, Japan aut NATIONALLICENCE 700 1- Yasuda Nobuko National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Miyasaka Atsushi National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 700 1- Kawasaki Shinji National Institute of Agrobiological Sciences, Tsukuba, Japan aut NATIONALLICENCE 700 1- Koizumi Shinzo National Agricultural Research Center, 3-1-1 Kannondai, 305-8666, Tsukuba, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/2(2006-04-01), 77-84 1345-2630 72:2<77 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer