caa a22 4500 467891052 CHVBK 20180406152749.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s10327-005-0253-9 doi (NATIONALLICENCE)springer-10.1007/s10327-005-0253-9 Susceptibility of hairy root lines of Brassica species to Plasmodiophora brassicae and in an in vitro subculture system [Elektronische Daten] [Takahiro Asano, Akiko Kodama, Koji Kageyama] To investigate the susceptibility of hairy root lines of Brassica species to Plasmodiophora brassicae, hairy roots were induced in a number of Brassica species with Agrobacterium rhizogenes. Turnip hairy root was highly susceptible to P. brassicae; infection rates were high and large galls formed. In contrast, the rates of root hair infection and gall formation on intact Brassica plants did not differ significantly from the control. To induce resting spore formation, turnip hairy roots were incubated at 15°, 20°, or 25°C after 3 weeks of incubation at 25°C. The number and fresh mass of the galls per hairy root were higher and formation of resting spores was greatest after a 7-week incubation at 20°C. To subculture P. brassicae using turnip hairy root, turnip hairy roots were reinoculated with resting spores and gall with resting spores then formed on the hairy roots. In this way, P. brassicae using hairy roots could be subcultured in vitro two or three times on three single-spore isolates of P. brassicae. This is the first report of in vitro subculture of P. brassicae using hairy root. The Phytopathological Society of Japan and Springer-Verlag Tokyo, 2006 Plasmodiophora brassicae nationallicence Hairy root nationallicence In vitro subculture nationallicence Brassica nationallicence Asano Takahiro River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut Kodama Akiko River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut Kageyama Koji River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut Journal of General Plant Pathology Springer-Verlag 72/2(2006-04-01), 85-91 1345-2630 72:2<85 2006 72 10327 https://doi.org/10.1007/s10327-005-0253-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10327-005-0253-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asano Takahiro River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut NATIONALLICENCE 700 1- Kodama Akiko River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut NATIONALLICENCE 700 1- Kageyama Koji River Basin Research Center, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan aut NATIONALLICENCE 773 0- Journal of General Plant Pathology Springer-Verlag 72/2(2006-04-01), 85-91 1345-2630 72:2<85 2006 72 10327 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer