caa a22 4500 46789339X CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2972-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-2972-x OsGLU1, A Putative Membrane-bound Endo-1,4-ß-D-glucanase from Rice, Affects Plant Internode Elongation [Elektronische Daten] [Hua-Lin Zhou, Si-Jie He, Yang-Rong Cao, Tao Chen, Bao-Xing Du, Cheng-Cai Chu, Jin-Song Zhang, Shou-Yi Chen] A dwarf mutant glu was identified from screening of T-DNA tagged rice population. Genetic analysis of the T1 generation of glu revealed that a segregation ratio of wild-type:dwarf phenotype was 3:1, suggesting that the mutated phenotype was controlled by a single recessive nuclear locus. The mutated gene OsGLU1, identified by Tail-PCR, encodes a putative membrane-bound endo-1,4-β-D-glucanase, which is highly conserved between mono- and dicotyledonous plants. Mutation of OsGLU1 resulted in a reduction in cell elongation, and a decrease in cellulose content but an increase in pectin content, suggesting that OsGLU1 affects the internode elongation and cell wall components of rice plants. Transgenic glu mutants harboring the OsGLU1 gene complemented the mutation and displayed the wild-type phenotype. In addition, OsGLU1 RNAi plants showed similar phenotype as the glu mutant has. These results indicate that OsGLU1 plays important roles in plant cell growth. Gibberellins and brassinosteroids induced OsGLU1 expression. In rice genome, endo-1,4-β-D-glucanases form a multiple gene family with 15 members, and each may have a distinct expression pattern in different organs. These results indicate that endo-1, 4-β-D-glucanases may play diverse roles in growth and developmental process of rice plants. Springer, 2006 endo-1,4-ß-D-glucanase nationallicence internode elongation nationallicence rice nationallicence T-DNA tagging nationallicence Zhou Hua-Lin National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut He Si-Jie National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Cao Yang-Rong National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Chen Tao National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Du Bao-Xing National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Chu Cheng-Cai National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Zhang Jin-Song National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Chen Shou-Yi National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 137-151 0167-4412 60:1<137 2006 60 11103 https://doi.org/10.1007/s11103-005-2972-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2972-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Hua-Lin National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- He Si-Jie National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Cao Yang-Rong National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Tao National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Du Bao-Xing National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chu Cheng-Cai National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jin-Song National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shou-Yi National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 137-151 0167-4412 60:1<137 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer