caa a22 4500 467893403 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2880-0 doi (NATIONALLICENCE)springer-10.1007/s11103-005-2880-0 The Maize Oil Yellow1 ( Oy1 ) Gene Encodes the I Subunit of Magnesium Chelatase [Elektronische Daten] [Ruairidh Sawers, Joanne Viney, Phyllis Farmer, Rhiannon Bussey, Gregory Olsefski, Katya Anufrikova, C. Hunter, Thomas Brutnell] Semi-dominant Oil yellow1 (Oy1) mutants of maize (Zea mays) are deficient in the conversion of protoporphyrin IX to magnesium protoporphyrin IX, the first committed step of chlorophyll biosynthesis. Using a candidate gene approach, a cDNA clone was isolated that was predicted to encode the I subunit of magnesium chelatase (ZmCHLI) and mapped to the same genetic interval as Oy1. Allelic variation was identified at ZmCHLI between wild-type plants and plants carrying semi-dominant alleles of Oy1. These differences revealed putative amino acid substitutions that could account for the alterations in protein function. Candidate lesions were tested by introduction of homologous changes into the Synechocystis magnesium chelatase I gene (SschlI) and characterization of the activity of mutant protein variants in an in vitro enzyme activity assay. The results of these analyses suggest that SsChlI protein variants containing the substitutions identified in the dominant Oy1 maize alleles lack activity necessary for magnesium chelation and confer a semi-dominant phenotype via competitive inhibition of wild-type SsChlI. Springer, 2006 AAA nationallicence chelatase nationallicence magnesium nationallicence maize nationallicence oil yellow1 nationallicence semi-dominance nationallicence AAA : ATPases associated with various cellular activities nationallicence Fe chelatase : ferrochelatase nationallicence Mg chelatase : magnesium chelatase nationallicence Mg proto IX : Mg protoporphyrin IX nationallicence proto IX : protoporphyrin IX nationallicence Sawers Ruairidh Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Viney Joanne Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut Farmer Phyllis Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Bussey Rhiannon Cornell University, 14853, Ithaca, NY, USA aut Olsefski Gregory Cornell University, 14853, Ithaca, NY, USA aut Anufrikova Katya Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Hunter C. Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut Brutnell Thomas Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 95-106 0167-4412 60:1<95 2006 60 11103 https://doi.org/10.1007/s11103-005-2880-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2880-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sawers Ruairidh Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Viney Joanne Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 700 1- Farmer Phyllis Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Bussey Rhiannon Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Olsefski Gregory Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Anufrikova Katya Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Hunter C. Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 700 1- Brutnell Thomas Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 95-106 0167-4412 60:1<95 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer