caa a22 4500 46789342X CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2417-6 doi (NATIONALLICENCE)springer-10.1007/s11103-005-2417-6 Molecular Cloning and Characterization of a Vacuolar H+-pyrophos-phatase Gene, SsVP , from the Halophyte Suaeda salsa and itsOverexpression Increases Salt and Drought Tolerance of Arabidopsis [Elektronische Daten] [Shanli Guo, Haibo Yin, Xia Zhang, Fengyun Zhao, Pinghua Li, Shihua Chen, Yanxiu Zhao, Hui Zhang] The chenopodiaceae Suaeda salsa L. is a leaf succulent euhalophyte. Shoots of the S. salsa are larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsa does not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na+ in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H+-PPase) from S. salsa. The SsVP cDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVP was induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsis overexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVP can increase salt and drought tolerance of transgenic Arabidopsis. Springer, 2006 euhalophyte nationallicence Suaeda salsa nationallicence H+-pyrophosphatase nationallicence overexpression nationallicence salt tolerance nationallicence drought tolerance nationallicence H+-PPase : H+-pyrophosphatase nationallicence H+-ATPase : H+-adenosine triphosphatase nationallicence RWC : relative water content nationallicence Guo Shanli Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Yin Haibo Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Zhang Xia Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Zhao Fengyun Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Li Pinghua Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Chen Shihua Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Zhao Yanxiu Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Zhang Hui Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 41-50 0167-4412 60:1<41 2006 60 11103 https://doi.org/10.1007/s11103-005-2417-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2417-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Shanli Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Yin Haibo Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Zhang Xia Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Zhao Fengyun Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Li Pinghua Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Chen Shihua Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Zhao Yanxiu Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Zhang Hui Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 41-50 0167-4412 60:1<41 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer