caa a22 4500 467893438 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2418-5 doi (NATIONALLICENCE)springer-10.1007/s11103-005-2418-5 Transcriptional Regulation of ABI3- and ABA-responsive Genes Including RD29B and RD29A in Seeds, Germinating Embryos, and Seedlings of Arabidopsis [Elektronische Daten] [Kazuo Nakashima, Yasunari Fujita, Koji Katsura, Kyonoshin Maruyama, Yoshihiro Narusaka, Motoaki Seki, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki] ABA-responsive elements (ABREs) are cis-acting elements and basic leucine zipper (bZIP)-type ABRE-binding proteins (AREBs) are transcriptional activators that function in the expression of RD29B in vegetative tissue of Arabidopsis in response to abscisic acid (ABA) treatment. Dehydration-responsive elements (DREs) function as coupling elements of ABRE in the expression of RD29A in response to ABA. Expression analysis using abi3 and abi5 mutants showed that ABI3 and ABI5 play important roles in the expression of RD29B in seeds. Base-substitution analysis showed that two ABREs function strongly and one ABRE coupled with DRE functions weakly in the expression of RD29A in embryos. In a transient transactivation experiment, ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly but these proteins activated the transcription driven by the RD29A promoter weakly. In 35S::ABI3 Arabidopsis plants, the expression of RD29B was up-regulated strongly, but that of RD29A was up-regulated weakly. These results indicate that the expression of RD29B having ABREs in the promoter is up-regulated strongly by ABI3, whereas that of RD29A having one ABRE coupled with DREs in the promoter is up-regulated weakly by ABI3. We compared the expression of 7000 Arabidopsis genes in response to ABA treatment during germination and in the vegetative growth stage, and that in 35S::ABI3 plants using a full-length cDNA microarray. The expression of ABI3- and/or ABA-responsive genes and cis-elements in the promoters are discussed. Springer, 2006 Arabidopsis nationallicence ABA nationallicence ABI3 nationallicence ABRE nationallicence DRE nationallicence germinating embryo nationallicence Nakashima Kazuo Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Fujita Yasunari Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Katsura Koji Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Maruyama Kyonoshin Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Narusaka Yoshihiro Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Seki Motoaki Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, 305-0074, Tsukuba, Japan aut Shinozaki Kazuo Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, 305-0074, Tsukuba, Japan aut Yamaguchi-Shinozaki Kazuko Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 51-68 0167-4412 60:1<51 2006 60 11103 https://doi.org/10.1007/s11103-005-2418-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2418-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakashima Kazuo Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Fujita Yasunari Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Katsura Koji Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Maruyama Kyonoshin Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Narusaka Yoshihiro Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 700 1- Seki Motoaki Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, 305-0074, Tsukuba, Japan aut NATIONALLICENCE 700 1- Shinozaki Kazuo Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, 305-0074, Tsukuba, Japan aut NATIONALLICENCE 700 1- Yamaguchi-Shinozaki Kazuko Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, 305-8686, Tsukuba, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 51-68 0167-4412 60:1<51 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer