caa a22 4500 467893446 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2941-4 doi (NATIONALLICENCE)springer-10.1007/s11103-005-2941-4 Characterization of cDNAs Encoding Two Distinct Miraculin-like Proteins and Stress-related Modulation of the Corresponding mRNAs in Citrus jambhiri Lush [Elektronische Daten] [Shintaro Tsukuda, Kenji Gomi, Hiroyuki Yamamoto, Kazuya Akimitsu] Two distinct full-length cDNAs from rough lemon that encoded miraculin-like proteins were isolated by random amplification of cDNA ends (RACEs), based on sequence information from subtractive PCR previously described, and designated as RlemMLP1 and RlemMLP2. The transcripts of both RlemMLP1 and RlemMLP2 were not detected in leaves, or stems but accumulated in fruits. Transcripts accumulated to higher levels in leaves after wounding, inoculation with conidia of Alternaria alternata, or treatment with methyl jasmonate vapors. Treatment with methyl salicylate antagonized the signaling pathway of wounding. Treatment with methyl salicylate at 2 h after wounding significantly reduced wounding-induced gene expression of both RlemMLP1 and RlemMLP2. Protein products of these genes were obtained by using a prokaryotic expression system, and had protease inhibitor activity. RlemMLP2, but not RlemMLP1, contained a thaumatin motif, and only RlemMLP2 showed anti-fungal activity against Alternaria citri. Cellular localization analysis with RlemMLP1 or RlemMLP2 fused to a green fluorescence protein gene following transient translation using a particle bombardment in onion cells indicated that both RlemMLP1 and RlemMLP2 were localized to the cytosol. These evidences revealed that rough lemon RlemMLPs are likely to have defensive function against pathogens at least when host cells are broken by their infections. Springer, 2006 Alternaria alternata nationallicence Citrus jambhiri nationallicence miraculin-like protein nationallicence protease inhibitor nationallicence rough lemon nationallicence thaumatin-like protein nationallicence BAEE : N-α-benzoyl-l-tyrosine ethylester hydrochloride nationallicence GFP : green fluorescence protein nationallicence GST : glutathione-s-transferase nationallicence IPTG : isopropyl-1-thio-β-d-galactoside nationallicence MeJA : methyl jasmonate nationallicence MeSA : methyl salicylate nationallicence MLP : miraculin-like protein nationallicence RACE : random amplification of cDNA ends nationallicence Tsukuda Shintaro Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Gomi Kenji Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Yamamoto Hiroyuki Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Akimitsu Kazuya Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 125-136 0167-4412 60:1<125 2006 60 11103 https://doi.org/10.1007/s11103-005-2941-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2941-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsukuda Shintaro Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Gomi Kenji Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Yamamoto Hiroyuki Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Akimitsu Kazuya Department of Life Sciences, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 125-136 0167-4412 60:1<125 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer