caa a22 4500 467893462 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-1673-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-1673-9 A Map of KNAT Gene Expression in the Arabidopsis Root [Elektronische Daten] [Elisabeth Truernit, Kirby Siemering, Sarah Hodge, Vojislava Grbic, Jim Haseloff] Homeodomain proteins are key regulators of patterning during the development of animal and plant body plans. Knotted1-like TALE homeodomain proteins have been found to play important roles in the development of the Arabidopsis shoot apical meristem and are part of a complex regulatory network of protein interactions. We have investigated the possible role of the knotted1-like genes KNAT1, KNAT3, KNAT4, and KNAT5 in Arabidopsis root development. Root growth is indeterminate, and the organ shows distinct zones of cell proliferation, elongation and differentiation along its longitudinal axis. Here we show that KNAT1, KNAT3, KNAT4 and KNAT5 show cell type specific expression patterns in the Arabidopsis root. Moreover, they are expressed in different spatially restricted patterns along the longitudinal root axis and in lateral root primordia. Hormones play an important role in maintenance of root growth, and we have studied their effect on KNAT gene expression. We show that KNAT3 expression is repressed by moderate levels of cytokinin. In addition, we show that the subcellular localization of KNAT3 and KNAT4 is regulated, indicating post-translational control of the activities of these transcription factors. The regulated expression of KNAT1, KNAT3, KNAT4 and KNAT5 within the Arabidopsis root suggests a role for these genes in root development. Our data provide the first systematic survey of KNAT gene expression in the Arabidopsis root. Springer, 2006 Arabidopsis nationallicence cytokinin nationallicence ethylene nationallicence homeodomain protein nationallicence KNAT nationallicence root meristem nationallicence GUS : β-glucuronidase nationallicence UAS : upstream activating sequence nationallicence ACC : 1-aminocyclopropane-1-carboxylic acid nationallicence IAA : indole-3-acetic acid nationallicence YFP : yellow fluorescent protein nationallicence GFP : green fluorescent protein nationallicence NLS : nuclear import signal nationallicence TALE : three amino acid loop extension, class of homeodomain proteins nationallicence Truernit Elisabeth Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut Siemering Kirby Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut Hodge Sarah Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut Grbic Vojislava Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut Haseloff Jim Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 1-20 0167-4412 60:1<1 2006 60 11103 https://doi.org/10.1007/s11103-005-1673-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-1673-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Truernit Elisabeth Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut NATIONALLICENCE 700 1- Siemering Kirby Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut NATIONALLICENCE 700 1- Hodge Sarah Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut NATIONALLICENCE 700 1- Grbic Vojislava Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut NATIONALLICENCE 700 1- Haseloff Jim Department of Plant Sciences, University of Cambridge, Downing Site, CB2 3EA, Cambridge, UK aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 1-20 0167-4412 60:1<1 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer